Therefore, this chapter just focuses on the critical components of conducting an in vivo comet assay test for regulatory submissions. The procedures presented include recommendations from the most recently adop
The comet assay is one of the standard tests for evaluating the genotoxic potential of a test item able to detect DNA strand breaks in cells or isolated nuclei from various tissues. The in vivo alkaline comet assay is part of the standard test battery, given in option 2 of the ICH ...
The comet assay: a sensitive genotoxicity test for the detection of DNA damage. Methods Mol. Biol. 291, 85–95 (2005). CAS PubMed Google Scholar Hartmann, A. et al. Recommendations for conducting the in vivo alkaline Comet assay. 4th International Comet Assay Workshop. Mutagenesis 18, 45...
The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the ...
Comet assay Alkaline comet assay was conducted using a comet assay kit (Biotechne, #4250-050-K, Minneapolis, MN, USA) following the manufacturer’s instructions. DNA was stained with Hoechst 33342 (Thermo Scientific, #62249) and fluorescence images were captured using a fluorescence microscope at...
Testicular cells were examined using the alkaline version of the Comet assay and the DNA damage was quantified as % tail DNA using a fully automatic scoring system. From the raw data 23 summary statistics were examined. A linear mixed-effects model was fitted to the summarized data and the ...
Fig. 1: An in vivo screen for genetic regulators of MN formation. a, Schematic of the in vivo micronucleus assay. Full protocol details have been described previously20. Data for aDscc1-KO mouse and a WT littermate control are shown. CD71, transferrin receptor; NCE, normochromatic erythrocyt...
Measurements using the alkaline comet assay predict bladder cancer cell radiosensitivity. Br. J. Cancer 2003, 89, 2271–2276. [Google Scholar] [CrossRef] [Green Version] Hinata, N.; Shirakawa, T.; Zhang, Z.; Matsumoto, A.; Fujisawa, M.; Okada, H.; Kamidono, S.; Gotoh, A. ...
All these factors may potentially affect the cells present inside the ECBs, but due to technical limitations, these issues cannot be studied in vivo. Thus, using an in vitro experimental set-up, the effect of these factors on hmNGF-releasing cells needs to be investigated. Our previous ...
489_ In Vivo Mammalian Alkaline Comet Assay-OECD Publishi 下载积分: 5000 内容提示: OECD/OCDE 489 Adopted: 29 July 2016 1 © OECD, (2016) You are free to use this material subject to the terms and conditions available at http://www.oecd.org/termsandconditions/. This Guideline was ...