First we verified that the Tie2-Cre transgene is able to induce efficient recombination of the floxed Alk5 gene in the AV canal by using the RT-PCR analysis and primers with target sequences flanking the floxed exon 3 (Fig. 1A). The wild-type sample demonstrated a single band of an ...
2001). The reduced expression of these genes in Wnt1-Cre;Alk5fl/fl embryos suggests that Alk5 acts as a master gene upstream of them to regulate mandible bone patterning (Fig. 10). The mandible patterning defects are directly caused by apoptosis in the proximal and aboral regions. Reduced ...
pressionofpairedboxgene8(Pax-8)orbonemorphogeneticproteinreceptortypeIA(BMPR-IA,alsonamed ALK-3)inratmyocytesbyRNAinterferenee.Methods:TheprimaryculturedH9C2(2-1)myocytesweredi- videdintofourgroups:shortinterferenceRNAtargetingPax一8(Pax-8siRNA)group,shortinterferenceRNA ...