AKT p308 immunohistochemistry in young rats.doi:10.1371/journal.pone.0046151.g003N. TiffanyBelayev LudmilaK. LarissaD. KristalZhang ChangdeG. NicolasPLOS ONE
LY294002 also inhibited elastase-induced p308 formation more in female smooth muscle cells than in males, and the corresponding cell media had less pro–MMP-9. AKT siRNA significantly decreased secretion of pro–MMP-9, as well as pro–MMP-2 and active MMP-2 from elastase-treated male rat ...
Note that under these conditions activation loop phosphorylation was not affected by knockdown of individual PHLPP isoforms (P308 panel). These data reveal that PHLPP2 controls the phosphorylation of the hydrophobic motif on Akt1 and Akt3 and PHLPP1 controls the phosphorylation of the hydrophobic ...
p389、S6K、AKT、p308-AKT 抗体购自Cell Signaling 公司;蛋白酶抑制剂购自Sigma 公司;雷帕霉素、 RAD001和AG1024购自Selleck公司。 1.2 短发夹RNA(shRNA)慢病毒重组质粒的构建 与鉴定 PC-1 的shRNA 靶点为GCTATCTCTACTTGTC TCC,以此设计shRNA 正义链序列(5'-GATCCGCT ...
Association of miRNA expression and DNA copy numberBlenkiron, CherieD Goldstein, LeonardP Thorne, NatalieSpiteri, InmaculadaChin, SuetFeungJ Dunning, MarkL BarbosaMorais, NunoE Teschendorff, AndrewR Green, AndrewO Ellis, Ian
The P308 protein shows characteristic upshift in the blots. We began by generating 3T3L1 TXNIP KO clones using CRISPR technology. After differentiating the KO cells into adipocytes, we re-expressed either the HA-TXNIP WT or a S308A mutant in these cells using lentivirus at a similar level as...