研究方法:miRNA-Seq、mRNA-Seq、 RIP-Seq、RIP-qPCR 研究摘要 非小细胞肺癌(NSCLC)是一种高致死性肿瘤,经常对靶向治疗产生耐药性。研究表明,TBK1磷酸化AGO2的S417位点(pS417-AGO2),通过增加microRNA诱导的沉默复合体(miRISC)的形成促进NSCLC的进展。临床NSCLC标本中高水平的pS417-AGO2与不良预后呈正相关。有...
实验方法:AGO2 RIP实验,miRNA测序 实验材料:VSMC与血小板共培养组(实验组),VSMC未处理组(空白组);模型小鼠等 研究内容 1.活化血小板促进人类VSMC分化增殖 首先,本篇文章作者以人类VSMC与活化血小板共培养的细胞为实验组(AP),未做处理的细胞为空白对照组(RP),进行qPCR和WB检测,结果显示共培养组细胞内与分化相关...
研究方法:miRNA-Seq、mRNA-Seq、RIP-Seq、RIP-qPCR 研究摘要 非小细胞肺癌(NSCLC)是一种高致死性肿瘤,经常对靶向治疗产生耐药性。研究表明,TBK1磷酸化AGO2的S417位点(pS417-AGO2),通过增加microRNA诱导的沉默复合体(miRISC)的形成促进NSCLC的进展。临床NSCLC标本中高水平的pS417-AGO2与不良预后呈正相关。有趣...
Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to...
The RIP/northern blotting analysis were performed as before. d Acetyl-mutants K493R or K720R, but not K355R of AGO2 decreases its interaction with pre-miR-19b1. AGO2-WT and mutants AGO2-K355R, -K493R, -K720R and -3KR were individually co-transfected with pre-miR-19b1 into ...
C Ago2 RNA immunoprecipitation (RIP) was performed, followed by qRT–PCR. A RIP assay with an Ago2 antibody was used to assess the binding ability between miR-144/451 and Ago2 mRNA in wt MEFs, TAp63−/− MEFs, or TAp63−/− MEFs transfected with a TAp63 expression construct. ...
Applications for which this antibody has been used, include immunofluorescence3, co-immunoprecipitation (IP)3, nuclear IP combined with quantitative Mass spectrometry (MS)26, Chromatin Immunoprecipitation (ChIP) with qPCR25 and RNA-IP for AGO2 associated RNAs (AGO2-RIP)25, 28. The suspicious ...
(B) RT-qPCR of endogenous VLDLR and RRAGD transcripts in both wildtype (white) and PUM double knockout (black) cells. (C) A model of the antagonistic effect of PUM on AGO2 regulation of VLDLR and RRAGD mRNAs. Full size image To determine whether a physical AGO2-PUM interaction could...
研究方法:miRNA-Seq、mRNA-Seq、 RIP-Seq、RIP-qPCR 研究摘要 非小细胞肺癌(NSCLC)是一种高致死性肿瘤,经常对靶向治疗产生耐药性。研究表明,TBK1磷酸化AGO2的S417位点(pS417-AGO2),通过增加microRNA诱导的沉默复合体(miRISC)的形成促进NSCLC的进展。临床NSCLC标本中高水平的pS417-AGO2与不良预后呈正相关。有...
The proliferation and differentiation of mammalian skeletal muscle satellite cells (MuSCs) are highly complicated. Apart from the regulatory signaling cascade driven by the protein-coding genes, non-coding RNAs such as microRNAs (miRNA) and circular RNAs