[Original String]: Klein, D; Günzburg, W.H; Salmons, B (2000) Gene transfer estimation by multiplex real-time PCR: Advantages and limitations Forgen-Symposium -Genes for Therapy and Prevention of Disease, Wildbad Kreuth, 2000.Klein, D...
The in-house VNTR method based on the protocol of the MIRU-VNTR typing manual was used with minor modifications: the amount of DNA polymerase used was 0.75 units per multiplex PCR, and the initial concentration of labeled primers was increased to 8 μm for locus 2165 and locus 2163b. The...
We compared the PKM mRNA levels in PkmM1/M1 or PkmM2/M2 mouse tissues using qRT-PCR (Figure 1B). To do so, we amplified PKM cDNA in a region upstream of the knockin point and common to PkmM1 or PkmM2 alleles. Overall, PKM mRNA levels in PkmM1/M1 or PkmM2/M2 mice were ...
a study from the University of Washington reported E gene [6] and N2 gene primer/probe sets (US Centers for Disease Control and Prevention) to have better sensitivity for SARS-CoV-2 detection assay [7]. A recent report by Anantharajah et al. evaluated RT-PCR assays utilizing WHO-recommende...
However, more exact signs of gene editing in plants are needed. For this, specially developed PCR methods and whole genome sequencing are often used [43]. Many investigators optimized methods of genome editing of fruit and berry crops by using the PDS gene. For example, the main parameters ...