metabolic engineering strategy involves terminating the host organism’s fatty acid elongation cycle with a thioesterase to produce a fatty acid that can then be converted to an acyl-CoA by an ATP-dependent ligase, and then finally converted to an alcohol by a FAR7,9,12. This approach was ...
In the process of lipid metabolism, both exogenous and endogenous FAs must be esterified to form bioactive fatty acyl-CoA through acyl-CoA synthetase (ACS) to enter the relevant metabolic pathways. This includes FA oxidation mediated by an intracellular β-oxidation system as well as the synthesis...