This study therefore compared the performance of the conventional acid guanidinium thiocyanate-phenol-chloroform (AGPC) extraction method to QIAamp Viral RNA Mini Kit (QIAGEN, Cat. No. 52906) and OxGEn RNA Kit (OxGEn Molecular Solutions, GE-009) to build an in-house RNA extra...
The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle... P Chomczynsk...
Acid guanidinium thiocyanate-phenol-chloroform (AGPC) is one of the oldest RNA extraction solutions, in use since 1987. It is known as a ready-made solution, sold under different brand names, and is typically the most expensive reagent in the RNA extraction process. In this article, we ...
Total RNA was extracted using phenol–chloroform extraction using TRIzol reagent (Invitrogen). Reverse transcription was performed with a First Strand cDNA synthesis kit (Roche) following the manufacturer’s instructions with random hexamers. Real-time PCR was performed with GoTaq qPCR Master Mix (Prom...
Phenol–chloroform extraction is widely used. The process consists of vigorous mixing of phenol–chloroform solution and sample followed by centrifugation [7]. Phenol does not completely inhibit RNase activity, and this characteristic enables isolation of nucleic acid by combination with chloroform and al...
Total RNA was isolated from in-vitro-derived cDC1 and cDC2 in the presence or the absence of 100 nM RA using phenol– chloroform extraction method provided by Immgen (http://immgen. org).11 RNA integrity was determined using an Agilent Bioanalyzer (Stanford PAN Facility, Stanford, CA). ...
Learn more about the different nucleic acid extraction methods and potential solutions for common problems during DNA or RNA extraction.
1.be free of phenol and protein contamination repeat 'chloroform extraction' will prevent phenol contamination 2.Care when removing the aqueous phase水相 3.Molecular weight (MW) of DNA, In emulsion乳化液step, Low-MW DNA can be achieved by vortexing涡流 ...
Med. Vol. 38(2), 134-143, 2006 Semi-quantitative RT-PCR Total RNA was extracted from young and senescent cells using an acid guanidinium thiocyanate phenol- chloroform extraction method (Chomczynski and Sacchi, 1987). In order to compare the amounts of mRNA in young and senescent cells, ...
Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction Anal. Biochem., 162 (1987), pp. 156-159 View PDFView articleGoogle Scholar 5 A. D'Agostino, M. Stefanini A rat spermatocyte surface protein is involved in adhesion of pachytene spermatocytes to ...