a改良后酚氯仿法提取到的DNA,具有较高纯度和较高回收率的特点,可能在微量检材DNA提取中的有一定应用价值。 After the improvement the phenol chloroform law withdraws DNA, has high-purity and the high returns-ratio characteristic, possibly in micro examines in the material DNA extraction to have certain...
ChloroformGuanidinesPhenolRNAHydrogen-Ion ConcentrationSince its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an ...
The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle... P Chomczynsk...
Basic Protocol: RNA ISOLATION WITH HOMEMADE ACID GUANIDINIUM THIOCYANATE-PHENOL-CHLOROFORM (AGPC) Here we describe the extraction of RNA using homemade AGPC, which is a versatile solution used to solubilize DNA, RNA, or protein. Proteins, such as RNases, are denatured by guanidinium thiocyanate...