Samples within each patient cohort were stained simultaneously with rabbit anti-ACE2 (1:100; Abcam ab15348) and mouse anti-acetylated α-Tubulin (ACTUB 1:300; Santa Cruz sc-23950) using the same master mix and identical incubation times under similar staining conditions described above. Isotype...
α-Tubulin was used as a loading control. P values were calculated using one-way ANOVA, *p < 0.05; **p < 0.01; ***p <0.001. Fig. S2. Molecular docking and simulation analyses of the interaction between ApoE and ACE2. A Molecular docking simulation of the SARS-CoV-2 RBD interacting ...
Samples within each patient cohort were stained simultaneously with rabbit anti-ACE2 (1:100; Abcam ab15348) and mouse anti-acetylated α-Tubulin (ACTUB 1:300; Santa Cruz sc-23950) using the same master mix and identical incubation times under similar staining conditions described above. Isotype...
2-Methoxyestradiol是一种microtubule和HIF-1抑制剂,在秋水仙碱位点或附近结合到tubulin,且抑制微管聚合,通过干扰正常微管功能而发挥作用。 注:本品仅可用于科研实验,严禁用于临床医疗及其他用途! CAS号:362-07-2 别名:2-ME2;NSC-659853 纯度:99.54% 分子式:C₁₉H₂₆O₃ 分子量:302.41 结构式: 储存...
α-tubulin; nauplius eye; neurogenesis Abstract. The formation of the central nervous system of the stomatopod crustacean Gonodactylaceus falcatus is described by means of antibody stainings against synapsin and α-tubulin. It is shown that the longitudinal fiber tracts of the ventral nervous syst...
In addition, we also used another reference control gene, α- tubulin to perform the qRT-PCR analysis in tomato. The results indicated there was negative correlation between expression of miR1918 and the expression of sly-TG2 and only sly-TG2 expression was down-regulated in all transgenic...
FtsZ is a GTPase with weak sequence homology to tubulins. The nature of FtsZ polymers in vivo is unknown, but FtsZ can form tubules, sheets and minirings in vitro. Here we report the crystal structure at 2.8 A resolution of recombinant FtsZ from the hyperthermophilic methanogen Methanococcus ...
The "housekeeping" protein α-tubulin was used as a loading and blotting control, with no variation observed in α-tubulin protein levels between groups (Supp.Fig. 4.a- d). Non-cropped versions of full blots are shown in Sup- plemental Figs. 2 and 3. To further evaluate kidney ...
GAPDH was used in qPCR as internal control and beta-tubulin was used in WB (1:5000, 66240-1-Ig from Proteintech) as an internal control. Flow cytometry analysis of human peripheral blood samples For surface staining, PBCs were incubated with fluorochrome-labeled antibodies specific for humans ...
Interestingly, although overall colocalization of vimentin-positive spots with ACE2 or Spike was moderate, a selective enrichment of the three proteins was detected at elongated structures, positive for acetylated tubulin and ARL13B. These structures, consistent with primary cilia, concentrated Spike ...