Anti-ARL13B C-5 (sc-515784) was from Santa Cruz Biotechnology, anti-ACE2 rabbit antibodies were purchased from Invitrogen and Abcam, anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451) was from Sigma and anti-SARS-CoV-2 Spike ...
Samples within each patient cohort were stained simultaneously with rabbit anti-ACE2 (1:100; Abcam ab15348) and mouse anti-acetylated α-Tubulin (ACTUB 1:300; Santa Cruz sc-23950) using the same master mix and identical incubation times under similar staining conditions described above. Isotype...
α-Tubulin was used as a loading control. P values were calculated using one-way ANOVA, *p < 0.05; **p < 0.01; ***p <0.001. Fig. S2. Molecular docking and simulation analyses of the interaction between ApoE and ACE2. A Molecular docking simulation of the SARS-CoV-2 RBD interacting ...
5.8S rDNA (ITS), and parts of the beta-tubulin (BenA), calmodulin (CaM), second largest subunit of nuclear RNA polymerase II (rpb2) and the large ribosomal subunit (LSU) were amplified by PCR using the primers and conditions previous described16,18. Sequencing was conducted in both ...
(F-10) sc-8002, Santa Cruz Biotechnology) at 1:200, cyclin D1 (CST-2922, Cell Signalling Technology) at 1:1000, PGR (NCL-L-PGR, Novocastra) at 1:500, FOXA1 (Abcam; ab 5089) at 1:1000, GAPDH [6C5] (Abcam; ab 8245) at 1:2000 and Tubulin (T-9026, Sigma- Aldrich) at 1...
(sc-23950)acetylated alpha Tubulin Antibody (6-11B-1) an Antibody bySanta Cruz Biotechnology Applications: IHC-IF(1:300) (sc-8020)Cytokeratin 8 Antibody (C51) an Antibody bySanta Cruz Biotechnology Applications: IHC (000664)C57BL/6J
ribosomal large subunit (LSU); three substitution site model with empirical-based frequencies (F) and I (TPM3 + F + I) for the translation elongation factor 1-a (tef1-a) gene, and Hasegawa-Kishino-Yano (HKY) with F, I, and G4 (HKY + F + I + G) for the β-tubulin (tub2)...
(1:2000; Santa Cruz, Dallas, TX, USA), ACE2 (SN0754; 1:500–1:1000; Invitrogen, Waltham, MA, USA), COX-2 (1:1000; Cell Signaling Technologies, Whitby, ON, Canada), Laminin A/C (1:1000; Cell Signaling Technologies, Whitby, ON, Canada) and β-Tubulin (1:50,000; Sigma, St....
β-tubulin expression Fwiagsuruese3d. EaxspprreostseioinncoofnitNenOtSmaanrdkegre.nReersautilotsnforof mNOonine oCf6thrarteegliinodmeapecneldlsenint ethxepeprriemsenctse aorfeCsMho5w44n.. (Da)enRseiptoremsentrtiactivvaelupersotaerienebxapnrdes soefdiNasOpSeorbcetanitnaegdesbyofWtheestienrtn...
MTT试剂为上海生博公司产品;多巴胺(dopamine,DA)为Sigma产品;NQO1抗体为Sigma产品;SF为LKT Laboratories产品。β-tubulin抗体为Millipore产品;脂质体转染试剂GenJetTMin vitro DNA Transfection Reagent为 SignaGen Laboratories产品。氯化硝基四氮唑蓝检测试剂盒为碧云天产品;蛋白浓度测定试剂盒为Bio-Rad产品;红外荧光Ⅱ抗为...