α-Tubulin was used as a loading control. P values were calculated using one-way ANOVA, *p < 0.05; **p < 0.01; ***p <0.001. Fig. S2. Molecular docking and simulation analyses of the interaction between ApoE and ACE2. A Molecular docking simulation of the SARS-CoV-2 RBD interacting ...
Aliquots were subjected to SDS gel electrophoresis in 10% polyacrylamide gels, electrophoretically transferred onto PVDF membranes (Millipore, USA), and probed with specific antibodies against ACE2 (ab59351, Abcam, USA), fibronectin (Sigma-Aldrich, USA), tubulin (Sigma-Aldrich...
5.8S rDNA (ITS), and parts of the beta-tubulin (BenA), calmodulin (CaM), second largest subunit of nuclear RNA polymerase II (rpb2) and the large ribosomal subunit (LSU) were amplified by PCR using the primers and conditions previous described16,18. Sequencing was conducted in both ...
the PVDF-membrane blots were blocked with 5% skimmed milk in PBST for 2 h at room temperature and then incubated 1 μg/mL anti-Flag mAb (Sigma, F1804), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (AntGene, ANT325) PAb or anti-β-tubulin (Immmuno Way, YM3030) mAb dilute...
The "housekeeping" protein α-tubulin was used as a loading and blotting control, with no variation observed in α-tubulin protein levels between groups (Supp.Fig. 4.a- d). Non-cropped versions of full blots are shown in Sup- plemental Figs. 2 and 3. To further evaluate kidney ...
(1:200; Sigma HPA000288), goat anti-ACE2 (1:100; R&D Systems AF933), rabbit anti-ACE2 (1:100; Abcam ab239924), mouse anti-ACE2 (1:200; R&D Systems MAB933), rabbit anti-ACE2 (1:100; Novus NBP2-67692), mouse anti-acetylated α Tubulin (1:300; Santa Cruz sc-23950), mouse...
Anti-ARL13B C-5 (sc-515784) was from Santa Cruz Biotechnology, anti-ACE2 rabbit antibodies were purchased from Invitrogen and Abcam, anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451) was from Sigma and anti-SARS-CoV-2 Spike ...
Samples within each patient cohort were stained simultaneously with rabbit anti-ACE2 (1:100; Abcam ab15348) and mouse anti-acetylated α-Tubulin (ACTUB 1:300; Santa Cruz sc-23950) using the same master mix and identical incubation times under similar staining conditions described above. Isotype...
Mouse acetylated tubulin Sigma T7451 Mouse Muc5AC Invitrogen MA5-12178 Mouse SARS-CoV-2 (COVID-19) spike antibody [1A9] Genetex GTX632604 Mouse beta-actin Santa Cruz sc-69879 Bacterial and virus strains SARS-CoV-2 SARS-CoV-2/human/Liverpool/REMRQ0001/2020 SARS-CoV-2 B.1.1.7 SARS-CoV...
Subsequently, the membrane was incubated with 1 μg/mL anti-FLAG mAb (Sigma, F1804) or anti-β-tubulin (ImmmunoWay, YM3030) mAb diluted in PBST containing 1% milk overnight at 4°C. After four washes with PBST, the blots were incubated with Horseradish peroxidase (HRP)-conjugated ...