Anti β-tubulin Antibody-HRP(细胞骨架内参抗体) 详细+ Anti Histone H3 Antibody-HRP(细胞核内参抗体) 详细+ RIPA裂解液(强,含抑制剂) RIPA 裂解液(强)对动物细胞胞膜、胞浆、胞核成分均有较强的裂解作用,是一种经典的细胞组织快速裂解液,可以从培养的哺乳动物细胞(包括铺板细胞和团状悬浮细胞)中高效裂解...
Anti β-tubulin Antibody-HRP(细胞骨架内参抗体) 详细+ Anti Hsp90 Antibody-HRP(全细胞内参抗体) 详细+ Anti GAPDH Antibody-HRP(全细胞内参抗体) 详细+ Anti Histone H3 Antibody-HRP(细胞核内参抗体) 详细+ RIPA裂解液(强,含抑制剂) RIPA 裂解液(强)对动物细胞胞膜、胞浆、胞核成分均有较强的...
In both cases, ROIs comprising the whole cell area or the area corresponding to the position of cilia, determined with an anti-acetylated tubulin antibody, were compared. Colocalization was analyzed using Fiji’s JACoP plugin and was presented as percentages of coincidental signals (Manders’ ...
Samples within each patient cohort were stained simultaneously with rabbit anti-ACE2 (1:100; Abcam ab15348) and mouse anti-acetylated α-Tubulin (ACTUB 1:300; Santa Cruz sc-23950) using the same master mix and identical incubation times under similar staining conditions described above. Isotype...
Tubulin (1:300; Santa Cruz sc-23950). Exposure times under fluorescence microscopy were identical for samples within the same cohort. Quantitation was performed in the FIJI package of ImageJ open source software. Binary masks were created by thresholding the anti-acetylated α-Tubulin channel using...
The results were normalized to the expression of a-tubulin. The data are expressed as the mean ± SD. E Cells were stained with ACE2 (green) antibody and counterstained with DAPI (blue) after transfection with 1 µg/ml Flag, ApoE2-Flag, ApoE3-Flag or ApoE4-Flag plasmids for ...
ARX788 is a next-generation ADC using a technology platform whereby a HER2-specific monoclonal antibody is conjugated with Amberstatin269, a potent cytotoxic tubulin inhibitor. Site-specificity, high homogeneity, and stable covalent conjugation of ARX788 lead to its slow release and prolongation of ...
α-tubulin; nauplius eye; neurogenesis Abstract. The formation of the central nervous system of the stomatopod crustacean Gonodactylaceus falcatus is described by means of antibody stainings against synapsin and α-tubulin. It is shown that the longitudinal fiber tracts of the ventral nervous syst...
the PVDF-membrane blots were blocked with 5% skimmed milk in PBST for 2 h at room temperature and then incubated 1 μg/mL anti-Flag mAb (Sigma, F1804), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (AntGene, ANT325) PAb or anti-β-tubulin (Immmuno Way, YM3030) mAb dilute...
GAPDH was used in qPCR as internal control and beta-tubulin was used in WB (1:5000, 66240-1-Ig from Proteintech) as an internal control. Flow cytometry analysis of human peripheral blood samples For surface staining, PBCs were incubated with fluorochrome-labeled antibodies specific for humans ...