absolute ethanol (99.7%), acetic acid (CH3COOH, 99.5%), sodium hydrogen phosphate, and trizma hydrochloride (Tris–HCl, ≥99.9%) were obtained from Chengdu XiYa Chemical Technology Co., Ltd. All other reagents were of analytical grade and used as received. Glass capillaries with an inner diame...
The absolute is then obtained by ethanol extraction of the concrete. Just as with cognac or davana, it exudes a fruity fragrance and also has sulfurous and animalic notes, giving it a lasting, sparkling character. Taste Description: black currant bud IFF Blackcurrant Bud Absolute Burgundy Odor ...
B. The histidine-tagged recombinant HBZ protein, which was produced by a wheat germ extract based cell free transcription and translation system (WEPRO7240H Expression kit; CellFree Sciences, Japan), was analyzed by SDS-PAGE. Gels were scanned and the levels of HBZ protein were quantified by ...
Gels were run for 30 min under 200 V, fixed for 30 min in 30% ethanol-7.5% acetic acid, and stained with Biosafe Coomassie blue (Bio-Rad). In the 25 kDa region of the gel encompassing toxins and QconCAT, protein bands were excised and were destained by repeated cycles of incubation...