absolute ethanol (99.7%), acetic acid (CH3COOH, 99.5%), sodium hydrogen phosphate, and trizma hydrochloride (Tris–HCl, ≥99.9%) were obtained from Chengdu XiYa Chemical Technology Co., Ltd. All other reagents were of analytical grade and used as received. Glass capillaries with an inner diame...
Sigma-Aldrich Cassis bourgens absolute, natural, FG sds Certified Food Grade Products SRS Aromatics BLACKCURRANT BUD ABS BURGUNDY (FG) SRS Aromatics BLACKCURRANT BUD ABS BURGUNDY Taytonn ASCC Blackcurrant Buds Abs Md 37% Tec Taytonn ASCC Blackcurrant Buds Absolute Burgundy The John D. ...
Gel pieces were then washed in HPLC grade water (Sigma-Aldrich) for 15 min before being dehydrated with 100% ACN. In-gel digestion was performed using 1:2 trypsin to protein ratio (sequencing grade modified trypsin, Promega) in 25 mm NH4HCO3 overnight at 37 °C. Peptides were extracted ...
N,N-Dimethyl-(2-aminoethyl) methacrylate (DMAEMA), methacryloyl chloride, anhydrous triethylamine, 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPDB-COOH), neutral aluminium oxide and 2-(methylamino)ethanol were purchased from Sigma Aldrich. The inhibitor removal for DMAEMA was performed ...
Needle emusfication was performed with 12 passes through a double luer-lock ended, 25 Gauge, 4-inch, micro-emulsifying needle (CAD7974 Sigma Aldrich). Sonication of cultures prior to FCM to assess effect on clump dispersal was performed by submerging 1 ml centrifuge tubes attached to a flotatio...
Analytical standard of meloxicam was purchased from Sigma-Aldrich (Bornem, Belgium), whereas meloxicam-13C6 IS was obtained from Alsachim (Illkirch Graffenstaden, France). All analytical standards were stored at 2–8 °C. For the analytical experiments, stock solutions (SS) of mavacoxib and ...
Materials: Dimethylformamide (DMF), tetrahydrofuran (THF), methanol (MeOH), ethanol (EtOH), and cyclohexane (CHX) were purchased from Sigma-Aldrich and dried and/or distilled according to conventional procedures. Orcinol, POCl3, MOMCl, NaH, n-BuLi, AcCl, Na2CO3, NaHCO3, H2O2 (30% w/w in...