为了研究Foxk1是否通过上调老年小鼠的糖酵解促进骨形成，研究人员将AAV9-Osterix-Foxk1和2DG通过尾静脉注射12月龄雄性小鼠，全身及单个器官显像显示GFP在后肢表达最高，肝脏表达较低，心、脾、肺、肾均未见表达；值得注意的是，IF染色显示GFP与股骨中的Osterix共定位，表明AAV9有效感染前成骨细胞。MicroCT分析显示2DG...
肾均未见表达；值得注意的是，IF染色显示GFP与股骨中的Osterix共定位，表明AAV9有效感染前成骨细胞。
2013. Systemic delivery of shRNA by AAV9 provides highly efficient knockdown of ubiquitously expressed GFP in mouse heart, but not liver. PLoS ONE 8, e75894.Piras BA, O'Connor DM, French BA. Systemic delivery of shRNA by AAV9 provides highly efficient knockdown of ubiquitously expressed GFP...
A pilot study in nonhuman primates (NHP) was conducted in which two Rhesus macaques receive d bilateral parenchymal infusions of adeno-associated virus serotype 9 encoding green fluorescent protein (AAV9-GFP) into each putamen. The post-surgical in-life was restricted to 3 weeks in order to ...
GFP distribution was visualization using the microscope. The level of GFP gene expression was also measured by Real-Time PCR. results indicated that transduction efficiency order in the heart was: AAV9>AAV8>AAV7>>>AAV2=AAV5. The transduction efficiency order in the liver was: AAV2>AAV5>AAV7...
用ADK9-1R抗体中和AAV9-GFP载体。HeLa细胞中显示AAV感染,感染后约48小时拍摄照片(GFP、CPE、合并)。随着ADK9-1R浓度的增加,中和作用增强。 抗AAV 9,ADK 9 - 1 R,小鼠重组抗体/anti-AAV9, ADK9-1R, mouse recombinant ab特点: 纯化、冻干 重组抗体 ...
Analysis of fetal gonads demonstrated GFP expression in female (but not male) germ cells, with low levels of integration-specific reads, without integration in select proto-oncogenes. These results suggest potential therapeutic benefit of AAV9 PSCGT for neuromuscular disorders, but warrant caution ...
为了研究Foxk1是否通过上调老年小鼠的糖酵解促进骨形成,研究人员将AAV9-Osterix-Foxk1和2DG通过尾静脉注射12月龄雄性小鼠,全身及单个器官显像显示GFP在后肢表达最高,肝脏表达较低,心、脾、肺、肾均未见表达;值得注意的是,IF染色显示GFP与股骨中的Osterix共定位,表明AAV9有效感染前成骨细胞。MicroCT分析显示2DG阻断...
基于以上特点,重组腺相关病毒已经成为科研和基因治疗中具市场前景的病毒载体之一。 病毒产品:维真AAV9-CMV-GFP 感染组织/器官:小鼠结肠组织 注射方法:尾静脉注射 病毒用量: 10E12vg 检测时间:注射后3周进行免疫组化 检测结果:AAV9感染结肠组织得到较为理想的结果...
为了研究Foxk1是否通过上调老年小鼠的糖酵解促进骨形成,研究人员将AAV9-Osterix-Foxk1和2DG通过尾静脉注射12月龄雄性小鼠,全身及单个器官显像显示GFP在后肢表达最高,肝脏表达较低,心、脾、肺、肾均未见表达;值得注意的是,IF染色显示GFP与股骨中的Osterix共定位,表明AAV9有效感染前成骨细胞。MicroCT分析显示2DG阻断...