基于以上特点,重组腺相关病毒已经成为科研和基因治疗中具市场前景的病毒载体之一。 病毒产品:维真AAV9-CMV-GFP 感染组织/器官:小鼠结肠组织 注射方法:尾静脉注射 病毒用量: 10E12vg 检测时间:注射后3周进行免疫组化 检测结果:AAV9感染结肠组织得到较为理想的结果...
and transduction efficiencies of scAAV9-GFP vectors carrying the Hb9 promoter enhancer elements and synapsin 1 (SYN1) promoter after delivery of the virus into neonatal mice via the cisterna magna by comparing them to the ubiquitous promoters CMV and chicken-β-actin fused to CMV enhancer (CAG)...
Intracisternal delivery of scAAV9-CMV-GFP mediates transgene expression in oligodendrocytes throughout the brain and the spinal cord white matter in injected young cats. Coronal sections of the white matter in the brain, that is, corona radiata from temporal and occipital lobes, (a–h) or in ...
A flag-tagged (N-terminal) open reading frame of mouse Rbm24 (ENSMUST00000037923;NCBIm37) was cloned into the pZac2.1 vector (under control of a CMV-promotor) and was subsequently used for AAV generation. AAVs were generated by the AAV Vector Unit at ICGEB Trieste (http://www.icgeb.org...
In contrast to the murine data, following intralingual delivery of AAV9-CMV-mCherry in adult Sprague-Dawley (SD) rats (1.42e10vg/渭L, 1.5 渭L or 7.0渭L), transgene expression in XII MNs was not detected using either fluorescence microscopy or antibodies against mCherry. Intralingual delivery...
We compared rAAV 5, 6, 8 and 9, containing either GFP or luciferase reporter genes driven by the CMV promoter. We demonstrated that although rAAV6 and 8 injected via renal vein transduced the kidney, transgene expression was mainly restricted to the medulla. Transgene expression was ...
To enhance targeting the transgene expression in CNS cells, self-complementary (sc) AAV9 vectors, scAAV9-GFP vectors carrying neuronal Hb9 and synapsin 1, and nonspecific CMV and CAG promoters were constructed. We demonstrate that synapsin 1 and Hb9 promoters exclusively targeted neuronsin vitro,...
[0050]图2是质粒pAAV‑GFP的环状图。 [0051]EGFP是编码修饰绿色荧光蛋白的序列, 多聚A是多聚腺苷酸化信号, ITR是腺相关病毒的反向末端重复, HBG内含子是人β‑珠蛋白内含子, CMV启动子是人巨细胞病毒启动子, AmpR是β‑内酰胺酶基因的序列,其提供大肠杆菌对氨苄青霉素的抗性, pUC起点是高拷贝细菌复制起...
Cultures of fibroblasts from three patients (GAN-T, GAN-S, and GAN-M) were infected with either vehicle, AAV2/JeT-GFP, AAV2/JeT-FLAG-GAN, or AAV2/CMV-GAN for 72 hr. Cells were then stained for vimentin (red), GFP (green), and DAPI to visualize the nuclei (blue). (A–C) Rep...
Immunofluorescence analyses on AAV2/9-CMV-eGFP ICV-treated newborn wild-type mouse brain. Newborn wild-type mouse were ICV injected with 2 × 1010 vg per mouse. Images showed AAV distribution at P60. (a–d) Double-staining with anti-GFP (green) and the nuclear marker TO-PRO3 (blue) ...