基于以上特点,重组腺相关病毒已经成为科研和基因治疗中具市场前景的病毒载体之一。 病毒产品:维真AAV9-CMV-GFP 感染组织/器官:小鼠结肠组织 注射方法:尾静脉注射 病毒用量: 10E12vg 检测时间:注射后3周进行免疫组化 检测结果:AAV9感染结肠组织得到较为理想的结果...
AAV-CAG-Cre-GFP (AAV9),即AAV9 serotype adeno-associated virus (AAV) expressing Cre-GFP,是一种可表达Cre和GFP融合蛋白的腺相关病毒,常用于注射小鼠等动物后进行基因编辑。CAG启动子是一种由巨细胞病毒(CMV)增强子和鸡β-肌动蛋白启动子组合而成的可以用于大多数基因编辑的高效启动子,驱动基因在真核细胞内...
[0050]图2是质粒pAAV‑GFP的环状图。 [0051]EGFP是编码修饰绿色荧光蛋白的序列, 多聚A是多聚腺苷酸化信号, ITR是腺相关病毒的反向末端重复, HBG内含子是人β‑珠蛋白内含子, CMV启动子是人巨细胞病毒启动子, AmpR是β‑内酰胺酶基因的序列,其提供大肠杆菌对氨苄青霉素的抗性, pUC起点是高拷贝细菌复制起...
and transduction efficiencies of scAAV9-GFP vectors carrying the Hb9 promoter enhancer elements and synapsin 1 (SYN1) promoter after delivery of the virus into neonatal mice via the cisterna magna by comparing them to the ubiquitous promoters CMV and chicken-β-actin fused to CMV enhancer (CAG)...
Intracisternal delivery of scAAV9-CMV-GFP mediates transgene expression in oligodendrocytes throughout the brain and the spinal cord white matter in injected young cats. Coronal sections of the white matter in the brain, that is, corona radiata from temporal and occipital lobes, (a–h) or in ...
AAV9 has significantly lower seroprevalence in the human population than other AAV serotypes, making AAV9 a desirable candidate for therapeutic applications.These AAV9 particles constitutively express ZsGreen under a CMV promoter. ZsGreen is a human codon-optimized variant of the green fluorescent ...
pZac2.1 vector (under control of a CMV-promotor) and was subsequently used for AAV generation. AAVs were generated by the AAV Vector Unit at ICGEB Trieste (http://www.icgeb.org/avu-core-facility.html) following a protocol as previously described13. AAVs encoding GFP were used as control....
specific cell types within the brain, we compared vectors expressing the GFP reporter gene under control of the truncated murine gfa2ABC1D promoter (GFA, astrocyte-specific), the human Synapsin promoter (Syn, neuron-specific) or the broadly active and robust hybrid CMV/CBA promoter as a ...
In contrast to the murine data, following intralingual delivery of AAV9-CMV-mCherry in adult Sprague-Dawley (SD) rats (1.42e10vg/渭L, 1.5 渭L or 7.0渭L), transgene expression in XII MNs was not detected using either fluorescence microscopy or antibodies against mCherry. Intralingual delivery...
基于以上特点,重组腺相关病毒已经成为科研和基因治疗中具市场前景的病毒载体之一。 病毒产品:维真生物AAV9-CMV-GFP 感染组织/器官:小鼠结肠组织 注射方法:尾静脉注射 病毒用量: 10E12vg 检测时间:注射后3周进行免疫组化 检测结果:AAV9感染结肠组织得到较为理想的结果...