There were no antibodies against GFP in ewes or lambs. Analysis of fetal gonads demonstrated GFP expression in female (but not male) germ cells, with low levels of integration-specific reads, without integration in select proto-oncogenes. These results suggest potential therapeutic benefit of AAV9...
[0057]AAV9‑01Mut‑GFP是指基于修饰腺相关病毒血清型9(rAAV9)的载体,其包含具有突 变F422W的修饰AAV9衣壳蛋白VP1。 [0058]AAV9‑02Mut‑GFP是指基于修饰腺相关病毒血清型9(rAAV9)的载体,其包含具有突 变I601M的修饰AAV9衣壳蛋白VP1。 [0059]AAV9‑03Mut‑GFP是指基于修饰腺相关病毒血清型9(...
In AAV9/JeT-GAN-infected cells, gigaxonin protein was increased over the endogenous levels of vehicle or scAAV9/JeT-GFP-treated cells (1e4, ∼20%, and 1e5, ∼31%; p < 0.05) (Figures 1D and 1E). For proof-of-concept studies, we used an AAV/JeT-FLAG-GAN vector that has an ...
42 Titers were calculated by qPCR with GFP primers (F5′-AGAACGGCATCAAGGTGAAC-3′, R 5′-GAACTCCAGCAGGACCATGT-3′), ITR primers (F 5′-GACCTTTGGTCGCCCGGCCT-3′, R 5′-GAGTTGGCCACTCCCTCTCTGC-3′), human growth hormone (hGH) primers (F 5′-TGGGAAGACAACCTGTAGGG-3′, R 5′...