Claudin-7 overexpression inhibits cell migration and invasion in NCI-H1299, a human NSCLC cell line that lacks endogenous claudin-7 expression. In the present study, we found that claudin-2 expression in human lung adenocarcinoma is higher than that in normal tissue and other lung carcinoma. We...
Our study aimed to investigate the effect of miR-324-3p on lung adenocarcinoma cell line A549 resistant to cis-diamminedichloroplatinum II (DDP, aka cisplatin). Methods The miR-324-3p expression levels in cisplatin-sensitive A549(A549) and cisplatin-resistant A549 (A549/DDP) cells were ...
Full size image The effect of betulinaldehyde on the intracellular autophagy level of A549 cells The intracellular autophagy level often changes according to the variation in the cell’s external environment and nutrition supply. Therefore, the level of autophagy in tumor cells is often comparatively...
Annexin V-PI staining (a, b) and caspase3 activity test (c) were performed to assess cell apoptosis. ***P < 0.001 Full size image TRIM65 induced the ubiquitination and degradation of TNRC6A in A549 cells TRIM65 is known to negatively regulate miRNAs by ubiquitinating TNRC6A31. Here...
The dashed line represents the upper threshold of ATM-S1981Por γH2AX IF level for 97% of interphase cells. Full size image Fig. 2 presents the quantitative data illustrating the CS-dose dependence of the induction of ATM-S1981P and γH2AX in A549 cells in relation to cell position in ...
Low dose α particle inducing radio-resistance in A549 cell line Firstly, we detected the induction of radio-resistance in human lung carcinoma A549 cell. Cells were irradiated with 5cGy α particle. 6 hours later, the irradiated cells were exposed to 75cGy α particle. The control group was...
mellea showed acute cell inhibition at the highest concentration compared to hot aqueous extract with rate of inhibition being less than 40% after 72 hr of treatment (Figure 1). However, the IC50 value for both D. mellea extracts were more than 1000 μg/ml on MRC5 cell line, ...
Adenocarcinoma, the most common form of lung cancer, is one of main human malignant tumors. In this paper, we focus on the effect of antitumor activity of cytokine-induced killer (CIK) cells on human lung adenocarcinoma cell line A549. CIK cells were obtained by inducing peripheral blood ...
Consequently, we evaluated the cytotoxic effect of grapefruit osthole against A549 lung cell line by MTT assay. The IC50of grapefruit osthole in the cell line was 188.5 μM after 48 h of treatment. On the other hand, the IC50of cisplatin, which was used as a reference drug, was 26.6 ...
The cleaved form of caspase-9 was found in BGT treated groups, however it was absent in the control cells (Fig. 4b and d). Overall, western blot analysis confirmed that BGT promotes intrinsic mode of apoptotic cell death in human NSCLC A549 cells. BGT induced mitochondrial membrane ...