DNA-binding proteinDNA-conjugated beadsDNA–protein interactions (DPIs)Pull-down assayDNA-protein interactions (DPIs) are critical to all living organisms, particularly in the regulation of gene expression, replication, packing, recombination, and DNA repair, as well as RNA transport and translation. ...
蛋白质互作技术英文是研究蛋白质间相互作用的技术英文表述。 其涵盖多种方法以解析蛋白质相互作用关系的英文术语。Yeast two - hybrid system(酵母双杂交系统)用于在酵母细胞中检测蛋白质互作。Co - immunoprecipitation(免疫共沉淀)可分离和鉴定相互作用的蛋白质复合物。Pull - down assay(蛋白质体外结合实验)能验证蛋...
Therefore, ARF6 activity was assessed using the protein-3 binding domain (PBD) of the golgi-associated, gamma adaptin ear-containing, ARF-binding protein 3 (GGA3) fused to GST (GST-GGA3-PBD) in a pull-down assay, which allows the detection of GTP-bound ARF6 in cells34 (Fig. 2b). ...
FAM13A pull down assay FAM13Acontains a Rho GTPase-activating protein-binding domain; it inhibits signal transduction and responds to hypoxia. Recent work by our research group indicates thatFAM13Ais involved in WNT/beta catenin pathway signaling23.FAM13Awas not mapped in the edge-weighted human i...
Using a pull-down assay, Pep-5 was shown to disrupt the Hsp90‒Cdc37 PPI, and isothermal titration calorimetry measured a slightly lower KD of 5.99 ± 0.8 μmol/L78. This was the first report of a synthetic polypeptide that was capable of disrupting Hsp90‒Cdc37 interactions, and ...
To further validate the CcpS-CpsB interaction, we performed a GST pull-down assay using the rCpsB-GST and the whole-cell lysates of S. suis cells. Western blot analysis showed that CcpS was retained with rCpsB-GST, but not GST (Fig. 4a). Next, to confirm whether CcpS can directly ...
Thus, the promoter pull-down assay was developed to fill this void. This method uses DNA as bait to capture proteins that bind to a specific promoter, such as transcription factors, from cellular lysates. Coupled with other experiments, the promoter pull-down assay vastly improves the repertoire...
(Fig.3a). A pull-down assay was used to further determine cirNRIP1-associated miRNAs via a specific biotin-labelled circNRIP1 probe. We first verified the significantly upregulated pull-down efficiency of the circNRIP1 probe in a circNRIP1 overexpression plasmid (pcDNA3.1) transfected into ...
Pull-up and pull-down resistors are not a special type ofresistorbut simply a constant value connected via, mainly, + 5V and pin or GND and pins. Resistor between pin and + 5V is called pull-up resistor, while the one between the pin and GND is a pull-down resistor. The default val...
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