While unfolding have been studied in detail at the protein level, a complete picture of the interplay between protein and surfactant in these processes is lacking. This gap in our knowledge is addressed in the current work, using the β-sheet-rich globular protein β-lactoglobulin (bLG). We ...
While much prior work has explored the constraints on protein sequence and evolution induced by physical protein-protein interactions, the sequence-level constraints emerging from non-binding functional interactions in metabolism remain unclear. To quantify how variation in the activity of one enzyme const...
The picture shows the red fluorescent protein TagRFP, which is able to photosensitize singlet oxygen, with an estimated quantum yield of ΦΔ≈0.004, as described by S. Nonell, C. Flors et al. on p. 161. This is the first estimation of a ΦΔ value for a GFP-like protein. More...
After doing some research and seeing TVP, textured vegetable protein, at our local co-op we concluded that it was the fastest and least expensive (we were college poor) way to consume high quantities of protein (Alex wasn’t even vegetarian at the time so it’s fascinating that he figured...
picture may be false – analysis of prehistoric remains suggests that Stone Age people were skilled in processing and cooking different kinds of seeds, even experimenting with differentflavours. Seeds are still an important part of ourdiets...
of secondary structures at the surface of each binding partner1. Such binding epitopes have inspired the design of molecules that mimic protein secondary structures to reproduce the projection of hotspot amino acids2,3. To date, peptidomimetic approaches focus on the mimicry of β-hairpins4, ...
Although there are still some technological issues that must be solved to produce a complete picture of protein phosphorylation, several technologies have been developed for the large-scale analysis of phosphoproteins, known as 'phosphoproteomics' [122]. Mass spectrometry analyses have identified thousands...
(HPAEC), and hydrophilic interaction liquid chromatography with mass spectrometry (HILIC-MS) is developed to obtain a more complete picture of the molecular weights (MW), composition, and salt-induced aggregation behavior of extracellular polymeric substances (EPS) produced by the algae Chlorella ...
), among which the protein encoded by PnGH1 had the highest match score of 509.45. Subsequent heterologous expression in Escherichia coli and purification via Ni2+ affinity chromatography allowed us to obtain the recombinant proteins PnGH1-3 (Fig. 3a, Supplementary Fig. 4). However, when these...
Picture shows a representative oocyte from 3 oocytes. (b) Uptake of GA3 by NPF3, NPF2.11 (GTR2) and non-injected oocytes at pH5 and pH7. Oocytes were incubated for 1 h in 100 μM GA3 (pH5 or pH7) (n=3). N: below detection limit. (c) Bioactive GA1, GA3 and GA4 uptake...