Dot-blotting — a novel screening assay for antibodies in hybridoma cultures 来自 Semantic Scholar 喜欢 0 阅读量: 37 作者:J Sternberg,P Jeppesen 摘要: A novel solid phase radioimmunoassay using nitrocellulose filter, is described. The method is of particular value in screening hybridoma cultures ...
Importantly, we also employed NB4 cells expressing a C-terminally truncated version of MYB (referred to as MYBΔ3) that is more active than the full-length protein40 and can be distinguished by western blotting from the wild-type protein, as shown in Fig. 4d. We reasoned that the ...
To quantify the expression of the CB1 receptor, the GLuc detection assay was performed 48 h after transfection. The culture medium was transferred to another well and each well containing transfected HEK293T cells was washed multiple times with PBS. A solution of 20 µM coelenterazine was dilut...
🟩 采用实时荧光定量聚合酶链反应(qPCR)、western blotting和免疫荧光染色法检测METTL14和FAT4在眼黑色素瘤细胞和组织中的表达。 🟩 建立细胞模型和异种原位移植 (Orthotopic Xenograft) 肿瘤模型以鉴定METTL14和FAT4在眼部黑色素瘤生长...
(5)HCT116细胞系中具有更高的METTL3表达,因此对其进行进一步分析。 2、转录组测序发现EphA2和VEGFA是影响血管发育的METTL3的直接靶点 (1)使用CRISPR-Cas9生成稳定的METTL3-KO(M3KO)HCT116细胞系,并使用qRT-PCR和western blotting验证,同时m6A dot blot证实M3KO之后,m6A水平被明显下调; ...
Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 ...
Immunoprecipitation and immunoblotting For pancreatic cancer organoid cells, cell lysate (25 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM CaCl2, 1%, 1 Triton X-100) with EDTA-free protease inhibitors (Biotool) was added to the dish, gently scraped with cell scraping and transferred to a 1.5 mL...
A. A. P. performed the biofilm assay and dot-blotting. D. N., H. Z., X. C. and X. L. collected x-ray diffraction data. Y. H., T. R., and Y. W. wrote the manuscript. Acknowledgments We thank Yan Zhao and staff members of beamline BL1A of Japanese KEK synchrotron and ...
c, Total RNA extracted from the indicated cell line, which was transfected with a DsiRNA specific to the target protein for 72 h and analysed by northern blotting with probes specific to the 5′-ETS, ITS1, and ITS2 sequences (Supplementary Table 8). As controls, cells were either ...
The resulting supernatant was used as a total fraction for western blotting (fibrillary tau tangles, cell membranes, and other cellular debris were removed in the pellet). In other experiments, proteins were extracted from mouse brain tissues with 2% SDS. 10–100 µg of the protein extract...