A Biophysical Perspective on Enzyme Catalysis. Even after a century of investigation, our understanding of how enzymes work remains far from complete. In particular, several factors that enable enzymes ... Pratul,K,Agarwal - 《Biochemistry》 被引量: 0发表: 2018年 加载更多来源...
A biophysical perspective on the cellulosome: new opportunities for biomass conversion. Curr. Opin. Biotechnol. 19:218-227.Ding SY, Xu Q, Crowley M, Zeng Y, Nimlos M, Lamed R et al (2008) A biophysical perspective on the cellulosome: new opportunities for biomass conversion. Curr Opin ...
l-asparaginase (l-ASNase) is an enzyme belonging to the class of amidohydrolases that catalyzes the hydrolysis of l-asparagine into l-aspartic acid and ammonia. It has been widely investigated as a biologic agent for its antineoplastic properties in treating acute lymphoblastic leukemia. The ...
Based on its clinical importance, fibrin is being investigated as a potentially valuable molecular target in the development of coagulation therapies. In this topical review, we summarize our current understanding of the coagulation cascade from a molecular, structural and biophysical perspective. We ...
As the presence of metal ions in the purified AbHpaI may not directly relate to the enzyme catalytic activity because their existence may depend on their availability in cells, we thus further investigated the effects of different metal ions on the catalysis of AbHpaI. First, the binding propert...
DNA polymerase couples chemical energy to translocation along a DNA template with a specific directionality while it replicates genetic information. According to single-molecule manipulation experiments, the polymerase-DNA complex can work against loads
The elements of the 3Rs-motivated testing strategy in the second “plane” [66], include uptake, biodistribution, biopersistence, biophysical interaction and cellular effects. These are highly consistent with mid-tiers of the GRACIOUS IATAs; however, the GRACIOUS Framework also seeks to incorporate...
In general, the catalysis mechanism of serine peptidases uses Ser195 to perform a nucleophilic attack on the carbonyl group of the peptide bond. The result is the cleavage of the peptide bond at the terminal carboxyl region of the target proteins37,38. The vast majority of serine peptidase ...
our research was mainly focused on experts and not on governmental staff, decision-makers, or public. The inclusion of these stakeholders would help to have another perspective on the SDGs importance and their relations with ES. Nevertheless, academics are those that are likely more aware of SDGs...
This work aimed to biochemically characterize the effects of the H163A mutation on the structure and ligand binding capabilities of this mutant in vitro. Our preliminary findings on the H163A mutant show significant biophysical and kinetic differences relative to the WT enzyme despite having a near...