Spread 50 µl from the vial on a labeled LB agar plate containing 50 µg/ml of kanamycin or 100µg/ml ampicillin and X-Gal. Be sure to include IPTG if you are using TOP10F´. Incubate plates overnight at 37ºC. Expected Results You shou...
The distribution of 37 tested NNJA peptides were also visualized in the global clustering map with the 7 NNJA peptides tested as siRNA conjugates labeled and shown in Supplementary Fig. S13. Figure 7 Global clustering analysis from all peptide sequences, with visualization for naïve library ...
infestans, naked dseGFP (dsRNA targeting the enhanced green fluorescent protein gene) was labeled with fluorescein to yield green fluorescence. After assembly with SPc, the fluorescence intensity of dseGFP was essentially unchanged (Supplementary Fig. 2). Confocal microscopy observations showed that ...
DON in cell-free supernatants after 4 days of incubation in TBI medium were harvested, extracted, and quantified by LC-MS. Data presented are the mean ± s.d. (n = 3). e Toxisome formation in the hyphae of ΔFgPCN, ΔFgADA3, and ΔFgGCN5 mutants and wild-type labeled ...
femtoCHROMO™-HRP with HRP labeled goat α-rabbit antibodyHRP型飞克级蛋白印迹膜检测 4000cm2 Protein Stains F新蛋白质考马斯亮蓝染色试剂盒 10minigels Streptavidin Resin 5ml Insect PE LB™昆虫细胞裂解液 250ml Phosphatase Assay kit磷酸酶活性分析 1000assays 2D-Xtract™2D电泳样品溶解液 50m...
Levels of fully labeled (13C) or unlabeled (12C) intracellular amino acids (from glucose catabolism) were quantified using a targeted metabolomics method.38 Growth on SM media supplemented with 12C- or 13C-glucose did not change cell growth parameters prior or post swap (Figures S1C and S1D...
The labeled BAC fragments were precipitated and analyzed with the ABI GS500-LIZ internal size standard (Applied Biosystems) on an ABI 3730XL DNA analyzer (Applied Biosystems) at the W.M. Keck Center at the University of Illinois. Data processing The fragment sizes in each BAC fingerprint ...
Samples were quenched with 50 mM NH4Cl, blocked in PBS with 1% BSA and immunolabeled with a-hPRXD6 1A11 (SantaCruz, 1:20) IgG1a monoclonal antibody in PBS with 1% BSA followed by a bridge step labelling with a polyclonal Rabbit Anti-...
Images were analyzed using the Photoshop software (Adobe), and numbers of probe-labeled cells and those of Nano orange-stained cells were counted on a computer screen. More than 100 Nano orange-stained cells in five sights were counted to obtain a ratio. Accession numbers The nucleotide ...
Developing programmable bacterial cell-cell adhesion is of significant interest due to its versatile applications. Current methods that rely on presenting cell adhesion molecules (CAMs) on bacterial surfaces are limited by the lack of a generalizable str