growth.• Stable, non-cytotoxic and cell non-adhesion surface • Easy to handle, compatible with liquid robotic system • Round bottom wells have high optical clarity making them suitable for bright field imaging and confocal microscopy. • Uniform single spheroid formation in each well ...
biosensor integrated into each well. It is an integral component of the EnSpire with label-free Corning ® Epic ® technology. The surface of each plate is tissue culture compatible and enables attachment and normal growth of adherent cells including native cells, recombinant / engineered cell ...
1. Glass-bottom 96-well plate (Cellvis, Catalog #P96–1.5HN or equivalent)" Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence I Miller, et al., Cell Reports, Volume 24, Issue 5, 31 July 2018, Pages 1105-1112 Quote: "96 well glass bottom plate, ...
This in vitro neuronal stretch injury model employs human induced pluripotent stem cell-derived neurons (hiPSCNs) in a 96 well format. Silicone membranes were attached to 96 well plate tops to create stretchable, culture substrates. A custom-built device was designed and validated to apply ...
Step.1 Cell seeding Cell was two-step diluted into desired density (16.7 cells/0.1 ml) in culture medium (SOP for two- step dilution please see Supplementary Information). The cell suspension was then sequentially introduced into culture wells (1,000 cells/well). After cells were successfully ...
1. Spheroid cell cultures may be seeded and grown up directly in the CellCarrier Spheroid ULA 96-well microplate provided in the kit in the same way you would seed cells into a standard 96-well microplate.2. For more details on seeding and growing spheroids see the “User’s Guide to ...
Reagents are sufficient for 400 assays in a 96-well plate format, but may be scaled to 48-well or 24-well plate formats. Related Products 1. : CytoSelect™ Cell Viability and Cytotoxicity Assay Kit 2. : CytoSelect™ MTT Cell Proliferation Assay Kit 3. : Total Cholesterol Assay Kit 4...
Thus, gp96 regulates B and T lymphopoiesis in a cell-intrinsic fashion. In addition, despite the presence of a WT competitor, gp96 LSK and progenitors were well sustained in the BM, and their percentage and number were consistently increased. Moreover, gp96 LSK and PROG were capable of ...
120 µL/well of pre-warmed medium was added subsequently to the apical chambers of the Transwell plate. Correction of raw data was accomplished by subtracting the electrical resistance as measured over an empty, cell-free insert. The final TEER values were obtained by multiplying with the inser...
75 μL into the cell culture insert on top of the DEs. While a standard 96-well receiver plate is normally used for the culture of reconstructed human epidermis models, ftSEs require a larger volume of medium per square centimeter culture area. Therefore, the described customized polycarbonate ...