经过辐照的 Gibco C57BL/6 小鼠胚胎成纤维细胞 (MEF) 是支持健康未分化胚胎干细胞 (ESC) 和诱导多能干细胞 (iPSC)(尤其是小鼠 ESC/iPSC)培养的饲养层细胞。由 MTI-GlobalStem 制造,等同于 MTI-GlobalStem 货号GSC-6002G 和 GSC-6202G,这些 MEF 细胞从同系交配 C57BL/6 小鼠中分离,通过辐照处理阻滞有丝...
简要描述:德国IKA欧洲之星20数显悬臂搅拌器实验室搅拌器,适用于常规搅拌,处理量高达15 l (H2O)。转速范围为0/30 – 2000 rpm,通过微处理器控制技术自动调节转速。安全回路设计可确保设备能在马达堵转或过载状态下自动停止运转 产品型号: IKA EUROSTAR 20 digital 所属分类:电动搅拌器 详细说明: 品牌 IKA/德国艾...
There is recent indirect evidence that T cells can inhibit B cell activity as well as enhance it 61 , and that they can enhance 62 and inhibit 63 the functioning of other T cells. It is not known if these interactions involve antigen bridging between the receptors of the interacting cells....
point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be ...
Meanwhile, MDA-MB-231 cells were seeded into 6-well cell culture plates, which are to be infected by the lentivirus. The resulting supernatant from the transfected HEK293T cells after 48 h became the lentivirus, which was then harvested and filtered through a 0.45 μm filter. Polybrene...
Cell counting Primary human lung fibroblasts were plated at a density of 5 × 104 cells per well in a 24 well plate. The next day cells were synchronized for 24 hours using DMEM/F12 medium supplemented with 0.1% FCS and antibiotics. Cells were stimulated with DMEM/F12 medium with 10...
Cell motility is a critical feature of invasive tumour cells that is governed by complex signal transduction events. Particularly, the underlying mechanisms that bridge extracellular stimuli to the molecular machinery driving motility remain partially understood. Here, we show that the scaffold protein CN...
MetaXpress 6软件指南：启用自适应获取功能说明书
2.3. In-vitro cell proliferation assays Mitochondrial activity, as a surrogate measure for cell viability, was measured using WST-1 assay. Cells were seeded at 3x103 cells/mL in a 96-well plate in triplicate. After 24 h the cells were treated with 0–105 nM of αvβ6-targeted SG3299 ...
Thermo Scientific Nunc Multidish, 24-Wells, Cell Culture Treated 142475 On the day of transfection, which should be 1 day following cell plating, perform the following steps, which have been optimized for a single well of a 24-well plate using Invitrogen Lipofectamine 3000 Transfection ...