经过辐照的 Gibco C57BL/6 小鼠胚胎成纤维细胞 (MEF) 是支持健康未分化胚胎干细胞 (ESC) 和诱导多能干细胞 (iPSC)(尤其是小鼠 ESC/iPSC)培养的饲养层细胞。由 MTI-GlobalStem 制造,等同于 MTI-GlobalStem 货号GSC-6002G 和 GSC-6202G,这些 MEF 细胞从同系交配 C57BL/6 小鼠中分离,通过辐照处理阻滞有丝...
point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be ...
There is recent indirect evidence that T cells can inhibit B cell activity as well as enhance it 61 , and that they can enhance 62 and inhibit 63 the functioning of other T cells. It is not known if these interactions involve antigen bridging between the receptors of the interacting cells....
Live cell imaging – confocal microscopy The day before doxycycline induction, 6 × 104 EphB6, EphB1, and/or ephrinB1-expressing MDA-MB-231 cells/well were seeded on a µ-Slide 8-well chamber coverslip (ibidi, Germany). Protein expression was induced by 20 ng/mL doxycycline for ...
MetaXpress 6软件指南：启用自适应获取功能说明书
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal interstitial lung disease. IPF is characterized by epithelial cell injury and reprogramming, increases in (myo)fibroblasts and altered deposition of extracellular matrix. The Wnt1-inducible s
Cell motility is a critical feature of invasive tumour cells that is governed by complex signal transduction events. Particularly, the underlying mechanisms that bridge extracellular stimuli to the molecular machinery driving motility remain partially understood. Here, we show that the scaffold protein CN...
OriCell TMC57BL 6 鼠脂肪膜肺源多植物肾上腺素受体1c（SSTR1c）表达的兔ADS OriCell TM C57BL/6 Mouse Adipose-derived Mesenchymal Stem Cells With GFP (ADSCs/GFP) Cat. No. MUBMD-01
Plate cells for transfection only 1 day before the experiment. Seeding cells for transfection The day before transfection, dissociate cells that are 80–90% confluent in a T-75 flask. Count the cells using standard trypan blue exclusion. ––Important: The cell number and concentration...
OriCell Strain C57BL 6 Mouse MSCs 产品说明书 OriCell TM Strain C57BL/6 Mouse Mesenchymal Stem Cells (MSCs)Cat. No. MUBMX-01001