(Product ## 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55kD using a mouse anti-human IgA monoclonal antibody (Product ## MA5-11208) diluted to a concentration of 0.5ug/ml in blocking buffer, overni...
(#9172 Cell Signaling technology), anti-phospho-Y701-STAT1 (#9167 Cell Signaling technology), anti-SOCS-5 (#sc-5607, Santa cruz biotechnol- ogy) anti-JEV NS1 (#ab41651; Abcam) and anti-β- tubulin (#ab6046; Abcam) primary antibodies were used after diluting in 5% BSA in TBST ...
图1 IFIT1在人胆管癌细胞及胆管癌组织的表达Figure 1 Expression of IFIT1 in human cholangiocarcinoma (CCA) cells and CCA tissues. A and B:the mRNA and protein expression levels of IFIT1 in CCA cells;C:the protein ...
图4 敲低mGluR5减轻6月龄5xFAD小鼠神经炎症反应Figure 4 Knockdown of mGluR5 reduced neuroinflammation in 6-month-old 5xFAD mice. A:the expression of GFAP, IBA-1, TNF-α and IL-1β in 6-month-old WT, mGluR5+/-...
Blotter (Product # 62288) and blocked with 5% milk in TBST for at least 1 hour at room temperature. FITC-BSA was detected at ~72 kD using a FITC monoclonal antibody (Product # MA5-14709) at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking pla...View ...
FH Kamal (Verified Customer) (01-26-2024) Empty heading Works well when diluted in 1X TBST. Applications:Western Blot Primary Antibody Dilution:1:2000 Cell Tissue Type:Mouse liver tissue
倒掉抗原修复液,TBST洗3遍,5分钟每次。封闭笔花圈,加0.6%过氧化氢(H202)封闭10分钟。TBST冲洗,5分钟后再次冲洗放5分钟。后加3%BSA封闭1小时。配制一抗,加一抗4度过夜。第二天,拿出标本室温下放置20分钟。TBST洗涤3次,15分钟每次。加入二抗,室温放置1小时。TBST洗三次,每次15分钟。DAB显色,注意把握时间。复...
Following an 1-hr block in 5%BSA in TBST buffer, membranes were incubated with primary antibody overnight, followed by horseradish-peroxidase-conjugated secondary antibodies. Proteins of interest were visualized following detection with ECL substrate (Pierce) and visualized by Amersham Gel Imager. ...
Membranes were blocked for 2 h at room temperature in TBST (20 mM Tris, 150 mM sodium chloride, 0.1% TWEEN-20, dH2O) with 5% non-fat milk. Membranes were incubated in primary antibody in TBST, 5%BSA overnight at 4 °C, anti-NMDAR2C (1 : 500; ab110, Abcam, ...
在tbst(tris-bufferedsaline-tween)中,使用5%牛奶对pvdf膜进行封闭1小时,随后在bsa/tbst稀释的3%一抗中轻柔摇晃孵化,维持4℃过夜。次日用pbs液清洗三次,在室温中用二抗对pvdf膜孵化1h,随后用增强化学荧光(enhancedchemiluminescence,ecl)试剂盒(millipore)及自动化学荧光曝光系统(tanon4200)对目标蛋白进行检测。