(#9172 Cell Signaling technology), anti-phospho-Y701-STAT1 (#9167 Cell Signaling technology), anti-SOCS-5 (#sc-5607, Santa cruz biotechnol- ogy) anti-JEV NS1 (#ab41651; Abcam) and anti-β- tubulin (#ab6046; Abcam) primary antibodies were used after diluting in 5% BSA in TBST ...
Blots were stained with Ponceau S to confirm uniform protein loading (Aldridge et al., 2008, Willenborg et al., 2005) before blocking in 5% BSA in TBST (10 mM Tris-HCl, pH 8, 100 mM NaCl, 0.05% Tween-20) for 1 hr. Blots were incubated with specific poly- or monoclonal antibodies ...
用 5%BSA-TBST 稀释一抗(FNDC5稀释为1∶1000,BMP2稀释为1∶1000,β-actin 1∶10000),4 ℃水平摇床孵育过夜。次日,TBST洗膜3次后相应二抗室温孵育 40分钟(1∶10000)。TBST洗膜3次后ECL显影,胶片曝光。 图3 7天时各组AKP染色情况Fig.3 AKP staining results in each group at the 7th day 3 统计学...
图1 IFIT1在人胆管癌细胞及胆管癌组织的表达Figure 1 Expression of IFIT1 in human cholangiocarcinoma (CCA) cells and CCA tissues. A and B:the mRNA and protein expression levels of IFIT1 in CCA cells;C:the protein ...
TBST冲洗,5分钟后再次冲洗放5分钟。后加3%BSA封闭1小时。配制一抗,加一抗4度过夜。第二天,拿出标本室温下放置20分钟。TBST洗涤3次,15分钟每次。加入二抗,室温放置1小时。TBST洗三次,每次15分钟。DAB显色,注意把握时间。复染细胞核(Hemotoxylin)约5-8秒。龙头水冲洗一次。醋酸(acetic acid)5秒,后氨水(...
【储存液】:0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 【产品应用】:not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user. 【保存条件】:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. ...
5%BSA封闭液, 37℃封闭NC膜1 h。分别加入稀释的一抗:proteasome 20S C2 Rabbit Ab (1:1000, Abcam), Rabbit Anti-β-actin(1:400, 北京博奥森), 4℃过夜。TBST洗膜5 min×3次, 加入稀释的辣根过氧化物酶标记的山羊抗兔IgG(1:8000, 北京博奥森), 37℃孵育2 h。TBST洗膜5 min×3次。等比例混合ECL...
(all from Cell Signaling) and anti-GAPDH (#MAB374, Millipore). For phospho-Syk detection, membranes were blocked and incubated with the primary antibody (#2710S, Cell Signaling) in 5% BSA in Tris-buffered saline with 0.1% Tween 20 (TBST). Densitometry analysis was performed using ImageJ ...
Membranes were blocked for 2 h at room temperature in TBST (20 mM Tris, 150 mM sodium chloride, 0.1% TWEEN-20, dH2O) with 5% non-fat milk. Membranes were incubated in primary antibody in TBST, 5%BSA overnight at 4 °C, anti-NMDAR2C (1 : 500; ab110, Abcam, ...
Afterward, the membrane was blocked using 5 % BSA in a Tris-buffered saline Tween-20 buffer (TBST) (20 mmol/L Tris–HCl, pH 7.4, 150 mmol/L NaCl, and 0.1 % Tween 20) for 1 hour at RT. The membranes were rinsed with TBST after blocking, and anti-EV-D68 3D (1:500,gift from ...