The invention provides fabG polypeptides and polynucleotides encoding fabG polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing fabG polypeptides to screen for antibacterial compounds.JIANZHONG HUANGDAMIEN MCDEVITT...
The invention provides fabG polypeptides and polynucleotides encoding fabG polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing fabG polypeptides to screen for antibacterial compounds.doi:US6110704 AJianzhong HuangDamien McDevittUS...
Efforts to understand the complex biology of A-encoded ketoacyl reductase mutants, a sub-population of E. coli BL21(DE3) host cells was selected from a small-opaque colony. This empirically selected host, named BL21(DE3)NH, allowed stable expression of these mutant proteins. Although the ...
ketoacyl-thioesters are unstable, the reactions contained the purifiedE. colifatty-acid synthetic enzymes; 3-ketoacyl-ACP reductase (FabG), 3-hydroxyacyl ACP dehydratase (FabA), and enoyl-ACP reductase (FabI) and NADH to convert the condensation product to the stable fully saturated form. By u...
The FabG 3-ketoacyl-acyl carrier protein (ACP) reductase of Escherichia coli has long been thought to be a classical member of the short chain alcohol dehydrogenase/reductase (SDR) family. FabG catalyzes the essential 3-ketoacyl-ACP reduction step in the FAS II fatty acid synthesis pathway. ...
Nucleic acids encoding 3-ketoacyl-ACP reductase from Moraxella catarrahalisThe present invention provides the genomic sequences of a library of purified nucleic acid molecules, or their complements, comprising the genome of proteins or portions thereof.KIM L. BERG...
R. Venkatesan, S. K. Sah-Teli, L. O. Awoniyi, et al., "Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase," Nat Commun, vol. 5, pp. 4805, 2014....
Tuberculosis (TB) still remains a worldwide health concern. Efforts to understand the complex biology of A-encoded ketoacyl reductase mutants, a sub-population of E. coli BL21(DE3) host cells was selected from a small-opaque colony. This empirically selected host, named BL21(DE3)NH, allowed ...