2. 该细胞贴壁生长,T25细胞培养瓶运输过程中经常出现大量细胞脱落并聚团的情况,细胞脱落建议按以下步骤处理: 收货发现细胞脱落聚集,可以将所有细胞收集起来,在50ml离心管内离心,上清保存备用,再用PBS重悬细胞,尽量吹散后离心弃上清。沉淀用1mL胰酶重悬,放37度消化2min,先轻轻吹散细胞,再加4ml左右完全培养基终止消化...
We have transfected 293 cells (lacking the FRT site) with the pcDNA5/FRT vector and have identified one potential second-site integrant after screening over 200 clones. DNA integrations can be detected by Southern blot. A single integrant will display a single band; double: two; triple: three...
培养基和添加剂Complete Growth Medium and Culture Conditions DMEM培养基(GIBCO,添加NaHCO3 1.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 供应限制Permits and Restrictions A。仅供研究之用。 REFERENCE Sena-Esteves M , et al. Single-step conversion of cells to retro...
将细胞培养瓶竖立放置，吸走培养基，如果培养基中的脱落细胞较多，说明细胞现在很容易脱落，只要加入1ml的PBS+EDTA(0.02%)，来回轻微晃动培养瓶直到细胞完全脱落，加入10mlMEM，混匀，不能吹打，分装2瓶。如果细胞没长满就脱落，则只需加入5mlMEM，不分装。如果培养瓶中的细胞贴壁较牢固，培养基上清没...
293T (A TCC® CRL-3216™) Derivation The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Comments This cell...
规格:1×10cells/T25培养瓶 分类:细胞系 别称Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo 型号:YLS-X0005 种属 人类 年龄(性别) 胎儿 组织来源 胚胎肾 生长特性 贴壁细胞 ...
5. Harvest cells, or culture supernatant, 48-72 h after transfection. For secreted protein, you can change media once (at day 3 or 4, save the collected sup) and give it another 3-4 days of culture. As long as the cells are alive, they produce proteins. However, the time when the...
5. Harvest cells, or culture supernatant, 48-72 h after transfection. For secreted protein, you can change media once (at day 3 or 4, save the collected sup) and give it another 3-4 days of culture. As long as the cells are alive, they produce proteins. However, the time when the...
The luciferase assay working solution component (A + B) was added directly to the culture medium using an equal volume to the volume of the culture medium, which was 50 µL. The cells were incubated for 30 min while gently rocking at room temperature. After that, luminescence was ...
Cav3 Cell Culture Protocol Provider From the laboratory of Edward Perez-Reyes, PhD, University of Virginia. References Perez-Reyes, E., Van Deusen, A., Vitko, I. Molecular Pharmacology of Human Cav3.2 T-Type Ca2+ Channels: Block by Antihypertensives, Antiarrhythmics, and Their Analogs....