Stem cellsHere, the authors identify four novel regulators of the 2-cell-like state, and thus totipotency, via unbiased CRISPR knockout screens and Dazl re-expression as a readout. They show that these factors act upstream of DPPA2 and DUX, and independently of p53....
A small subgroup of embryonic stem cells (ESCs) exhibit molecular features similar to those of two-cell embryos (2C). However, it remains elusive whether 2C-like cells and 2C embryos share similar epigenetic features. Here, we map the genome-wide profiles of histone H3K4me3 and H3K27me3 ...
Here, we map the genome-wide profiles of histone H3K4me3 and H3K27me3 in 2C-like cells. We found that the majority of genes in 2C-like cells inherit their histone status from ESCs. Among the genes showing a switch in their histone methylation status during 2C-like transitions, only a...
Primordial germ cells (PGCs) are common ancestors of all germline cells. However, mechanistic understanding of how PGC specification occurs is limited. Here, we identified transcription factor CP2-like 1 (Tfcp2l1), an important pluripotency factor, as a pivotal factor for PGC-like cell (PGCLC...
Mouse embryonic stem cells (ESCs) sporadically transit into an early embryonic-like state characterized by the expression of 2-cell (2C) stage-restricted transcripts. Here, we identify a maternal factor—negative elongation factor A (NELFA)—whose heterogeneous expression in mouse ESCs is coupled ...
表达宿主:E.coli/Cells 内毒素:经LAL检测验证,小于0.1ng/ul(0.1EU/ug) 纯化方法:亲和纯化。 应用范围:用于细胞培养的刺激剂;用于ELISA、CLIA、WB、IHC等免疫检测。 产品纯度:不低于总蛋白的90%。 储存方法:冻干蛋白存放在-20℃保存1年;复溶后蛋白溶液在无菌条件下-20℃至-80℃保存3个月;复溶后蛋白溶液在...
www.nature.com/cddis ARTICLE OPEN Bcl-2 inhibition combined with PPARα activation synergistically targets leukemic stem cell-like cells in acute myeloid leukemia Chendi Xie1,2,3,6, Hui Zhou1,2,6, Dongmei Qin1,2, Huijian Zheng4, Yuanfang Tang1,2,3, Wenjuan Li1,2,3, Jie Zhou1,2, ...
CD3 CD4+ cells with a Th2-like pattern of cytokine production in the peripheral blood of a patient with cutaneous T cell lymphoma[J].Leukemia:Official Journal of the Leukemia Society of America,Leukemia Research Fund,U.K 1997,11.Brugnoni D, Airo P, Tosoni C, et al. 1997. CD3-CD4+ ...
Cells were fixed and stained for MTs (blue) and actin (red). White arrowheads indicate co-aligning MTs and actin structures. Scale bar indicates 10 μm. Full size image G2L proteins have previously been shown to contribute to the regulation of cell division. G2L3 knockout mice die early...
The B cells expressed MHC classes I and II; the costimulatory molecules CD40, CD80, and CD86; the adhesion molecule CD54; and the B Discussion The capacity of exosomes to present allergen-derived peptides and to activate allergen-specific T cells was investigated. To obtain sufficient quantities...