CAGGAAGTAAGATGCCTGGAAC 下游:TGCAGCAGTACCAAGTTTAGTG 上游:AAGAGCAGCATGACCTCTCAC 下游:CTGCCTCAAGTCAGTGCCT 上游:AGAGAGCGATGCAGGAAACTT 下游:AAGGATGCCCACATCTTTTGG 上游:AAGAGGGATGCTGCCCTTAC 下游:TACGGCCAAATCCGTTCACA 筛选标准.使用 Cytoscape 软件构建"基因-中医症 状/现代医学症状"和"基因-中药"关联...
FDX1 上游:CCTCTTTGGAGTCTCTCGCGG 下游:CAGCCCAACCGTGATCTGT DLD DLAT GAPDH 上游:CTCTTGCTTTGGAATATGGAGC 下游:AAAAGCTTCTGATAAGGTCGGA 上游:ACTCCCCAGCCTTTAGCTC 下游:CAATCCCTTTCTCTACTGCCAAC 上游:GGAGCGAGATCCCTCCAAAAT 下游:GGCTGTTGTCATACTTCTCATGG dehydrogenase,GAPDH)为内参按 2−∆∆Ct 法分析....
and cDNA was synthesised with a Rever Tra Ace qPCR kit (Catalogue Number FSQ-201, TOYOBO, Japan). qRT-PCR was conducted using SYBR Green Real-Time PCR Master Mix (Catalogue Number QPK-201, TOYOBO, Japan) on a CFX96 Touch Detection System (Bio-Rad, USA). The 2−∆∆Ctmethod...
The expression levels ofD2S(forward primer sequence: 5′-GTCTCCTTCTACGTGCCCTT-3′; reverse primer sequence: 5′-GGGCAGCCTCCTTTAGTGGA-3′) andD2L(forward primer sequence: 5′-GCAAGCGAGTCAACACCA-3′; reverse primer sequence: 5′-CCTCGGGGTGAGTACAGTTG-3′) were determined by quantitative re...
The human ACE2 transcript variant 2 was amplified using the OriGene primer set Forward: 5’-TCC ATT GGT CTT CTG TCA CCC G-3’ and Reverse: 5’-AGA CCA TCC ACC TCC ACT TCT C-3’. HPRT was used as the control housekeeping gene using primers, Forward: 5’-TCA GGC AGT ATA ATC CAA...
The sequences of the BAX primers were: 5’-TGGCAGCTGACATGTTTTCTGAC-3’ and 5’-TCACCCAACCACCCTGGTCTT-3’ , while the sequences of the GAPDH primers were: 5-ATGGGGAAGGTGAAGGTCG-3’ and 5’-GGGTCATTGATGGCAACAATATC-3’ , resulting in a 107-bp PCR amplicon. qRT-PCR was ...
根据 MIP-2 基因 ORF 序列设计扩增引物 MIP-2- text-F(5'-CAGAGTCCTGATGCTCCTTGC-3')和 MIP- 2-text-R(5'-CCAGTCTTATCTTGGGGTCGA-3'),预计 扩增片段长度为 224 bp.以β-actin 基因(AB020884) 为内参设计引物,pActin2(+)5'-TCGTGCGTGAC- ATCAAGGAG-3' 和 pActin2(-)5'-CGCACTTCATGA- TG...
(F: 5-’ TCAGGCGTCTGTAGAGGCTT-3’R: 5-’ ATGCACATCCTTCGATAAGACTG-3′), ZEB1 (F: 5-’ GATGATGAATGCGAGTCAGATGC-3’R: 5-’ ACAGCAGTGTCTTGTTGTTGT-3′), ZEB2 (F: 5-’ CAAGAGGCGCAAACAAGCC-3’R: 5-'GGTTGGCAATACCGTCATCC-3′), Vimentin (F: 5-’ GACGCCATCAACACCGAGTT-3’R...
ifseraetntrciebueftfeedcttsowthheicfhacctatnhaetxtphleailnowthliesvdeelcorfepas-epoinlaχrixzIxTezAd, see Supplementary Fig. S5(a). The large variation generated SH power for s-polarized fundamental bofeaχmzIxTxiAs close to the detection limit. Note that the visibility of the fringes ...
Second, weak localization is also dethuwhxpeiigectrthhliusuvrcFdednhdSiefesdfaamei,ngrnbeneiesensagocttsatauhsrtuirohovespaweepcyLρn.aeTMnsxix.onb(BRTtetee,h)scbidetacuuteoithnnsnteethsfioeretftiohreMsmledetRds.ooTitforFoheFfiicgFSRrt.edi a1r3o4,nm,en3ni2.seioioI-gsnfltaiprcqtotouiu...