2–∆∆Ct 是一种计算实时荧光定量PCR（qPCR）时候计算样品中某个基因的相对表达量的方法。该法由K...
在每20μL的反应中,使用PowerUp SYBR Green Master Mix(Thermo Fisher Scientific,A25778)通过定量实时聚合酶链反应(qRT-PCR)扩增10ng cDNA,并使用7500快速实时PCR系统(应用生物系统)测定mRNA表达。用△Ct法将mRNA水平标准化为甘油醛3-磷酸...
Based on characterization, the minimum and maximum values refer to sample tests and represent the mean value plus or minus three times the standard deviation (mean±3Σ). 5.1.2 Typical values Unless otherwise specified, typical data are based on TA=25°C, V33=3.3V (for the 3.0V≤V33≤3.6...
输入数据需要有至少三列，Sample、Detector、Ct，分别对应样本名、基因名、Ct值。可以有更多列数据，顺序...