1H-15N HSQC spectra showing the crosspeak assignments of MPS-1(134–256) in the absence or presence of DPC micelles.Pan, Li
The solid state NMR 2D1H-15N HSQC spectra of fully protonated GFP-R5 and catMMP12-R5 (Fig. 1) could be acquired in 4 h 34′ and 10 h 36′ respectively on 3 mg of sample, including the bioinspired-silica matrix. The comparison with solution state 2D1H-15N HSQC is immediate an...
It is shown that in certain circumstances the sensitivity of nitrogen-15-proton gradient-selected HSQC spectra of proteins dissolved in H O is compromised due to exchange between the NH and the water protons. The loss in sensitivity arises because, at the start of the relaxation delay, the mag...
5. 2D strips from a 3D 'H-I'N NOESY-HSQC spectrum of uni- formly '5N-labeled wild-type Cp ZnRd showing sequential C,, H(i)- NH(I + 1) NOE connectivities for residues Phe 49-Clu 54. The spectrum was obtained at 25 "C with a mixing time of 150 ms. Figure 6, indicate...
3D 15N- or 13C-filtered NMR spectra provide much better resolution, but data acquisition requires long times. 2D 1H–15N or 1H–13C NMR spectra, such as HSQC, provide adequate resolution within a reasonable time for data collection. Therefore, they have been commonly used to identify drug-...
现有PANSY技术主要围绕平行采集高频与低频核的二维异核相关,例如1HX与19FX HSQC/HMBC(X为13C,15N等低频核)[19]。但关于1H/19F这两种高频核的二维同核相关平行采集却未见报道。近年来 随着氟工业的发展,运用NMR对有机氟化合物进行研究受到广泛关注[20]。其中,1H1H COSY和19F19F COSY是对有机氟化合物结构鉴定...
Fig. 2. Natural abundance 2D 1H,13C-HSQC spectra of Pin1 aromatic region. a) Spectrum of uniformly 15N-labeled Pin1. Cross peaks from this sample were set as the reference for quantitation of isotope incorporation in deuterated samples (without accounting for spectral changes caused by deuterati...
摘要: 米菲司酮的1HNMR及13CNMR研究孔漫1)贺文义韩广甸王映红(中国医学科学院中国协和医科大学药物研究所,北京100050)米菲司酮(Mifepristone,RU486)11β-〔4-(N,N-二甲氨基)苯基〕-17α-(丙炔-1)-Δ4,9-雌... 展开 关键词: 米非司酮 NMR 1HNMR 13CNMR 药物物理学 被引量: 4 年份: 1996 收藏...
Predict 1D31P and19F NMR and 2D1H–13C HSQC-DEPT and long range15N–1H HMBC NMR spectra for a structure Predicted NMR spectra for peptides Calculate 2D NMR spectra for1H,13C, and15N nuclei Predicted1H NMR spectrum of a four component mixture ...
Sorting nexin 3 (SNX3) belongs to a sub-family of sorting nexins that primarily contain a single Phox homology domain capable of binding phosphoinositides