1994. Minimizing sensitiv- ity losses in gradient-selected 15N-1H HSQC spectra of proteins. J. Mag. Re- son. A 107:178-84Stonehouse, J., Shaw, G. L., Keeler, J. & Laue, E. D. (1994). Minimizing sensitivity losses in gradient- selected 15N-1H HSQC spectra of proteins. J. Magn...
15N Aβ40是有O4的titrated,并且1H-15N HSQC范围被记录。 翻译结果3复制译文编辑译文朗读译文返回顶部 15N] Aβ40 滴定 O4,与及录得 1 H-15N 异谱。 翻译结果4复制译文编辑译文朗读译文返回顶部 15N]aβ40是与o4 titrated,1H-15N hsqc光谱的记录。
It is shown that in certain circumstances the sensitivity of nitrogen-15-proton gradient-selected HSQC spectra of proteins dissolved in H O is compromised due to exchange between the NH and the water protons. The loss in sensitivity arises because, at the start of the relaxation delay, the mag...
The solid state NMR 2D 1H-15N HSQC spectra of fully protonated GFP-R5 and catMMP12-R5 (Fig. 1) could be acquired in 4 h 34′ and 10 h 36′ respectively on 3 mg of sample, including the bioinspired-silica matrix. The comparison with solution state 2D 1H-15N HSQC is immediate...
The 1H, 15N-HSQC spectra on hPARP14 MD2 (residues 999–1191 of the full-length protein) shows a great resonance dispersion (HNs) for both forms of the protein as shown in Fig. 2a (free) and Fig. 2b (ADPr bound). Fig. 2 700 MHz 1H, 15N-HSQC assigned spectrum of the a 0.6 mM...
Results and Discussion The solid state NMR 2D 1H-15N HSQC spectra of fully protonated GFP-R5 and catMMP12-R5 (Fig. 1) could be acquired in 4 h 34′ and 10 h 36′ respectively on 3 mg of sample, including the bioinspired-silica matrix. The comparison with solution state 2D ...
Sequence- specific assignments were achieved using 2D 15N-1H HSQC, 3D HNCA, 3D HN(CO)CA, 3D HNCACB, 3D CBCA(CO)NH, 3D C(CO)NH and 3D HNCO experiments. All spectra were processed with nmrPipe and analyzed with NMRView. In addition to the classical 3-dimensional approach, othe...
Isotope-labeled fibrillated FAS1-4 A546T was dissolved in a ternary mixture 95/4/1 v/v/v% dimethylsulfoxide/water/trifluoroacetic acid, to obtain and assign a reference 2D 1H–15N HSQC spectrum for the H/D exchange analysis. Here, we report the near-complete assignments of backbone and ...
Predict 1D31P and19F NMR and 2D1H–13C HSQC-DEPT and long range15N–1H HMBC NMR spectra for a structure Predicted NMR spectra for peptides How It Works NMR Predictions in a Few Simple Steps Request a Consultation or Demo 1Import a structure from a file, or draw the molecule(s) in th...
NMR spectra were processed using the program NMRPipe (Delagio et al., 1995) and analyzed using the program SPARKY (Goddard et al.). The assignments 3 were further confirmed by sequential NOEs in the 15N-edited NOESY-HSQC and 13C-edited NOESY-HSQC spectra. Extent of assignments ...