(1994). Minimizing sensitivity losses in gradient- selected 15N-1H HSQC spectra of proteins. J. Magn. Reson. A, 107, 178 ± 184.Stonehouse, J., Shaw, G. L., Keeler, J. & Laue, E. D. (1994). Minimizing sensitivity losses in gradient- selected 15N-1H HSQC spectra of proteins. J...
It is shown that in certain circumstances the sensitivity of nitrogen-15-proton gradient-selected HSQC spectra of proteins dissolved in H O is compromised due to exchange between the NH and the water protons. The loss in sensitivity arises because, at the start of the relaxation delay, the mag...
NMR spectra were collected at 303 K on either a Varian Inova 600 MHz AS (CeBiME-LNLS) or on a Bruker Avance 700 MHz (University of Rome II) spectrometers. The 15N edited HSQC spectrum and the HNCA, HN(CO)CA, HNCO, HN(CA)CO, HNCACB and CB- CACONH 3D triple resonance experiments ...
The solid state NMR 2D 1H-15N HSQC spectra of fully protonated GFP-R5 and catMMP12-R5 (Fig. 1) could be acquired in 4 h 34′ and 10 h 36′ respectively on 3 mg of sample, including the bioinspired-silica matrix. The comparison with solution state 2D 1H-15N HSQC is immediate...
The 1H, 15N-HSQC spectra on hPARP14 MD2 (residues 999–1191 of the full-length protein) shows a great resonance dispersion (HNs) for both forms of the protein as shown in Fig. 2a (free) and Fig. 2b (ADPr bound). Fig. 2 700 MHz 1H, 15N-HSQC assigned spectrum of the a 0.6 mM...
Predict 1D 31P and 19F NMR and 2D 1H–13C HSQC-DEPT and long range 15N–1H HMBC NMR spectra for a structure Predicted NMR spectra for peptides Calculate 2D NMR spectra for 1H, 13C, and 15N nuclei Predicted 1H NMR spectrum of a four component mixture Predicted 1H NMR spectrum showing ...
Isotope-labeled fibrillated FAS1-4 A546T was dissolved in a ternary mixture 95/4/1 v/v/v% dimethylsulfoxide/water/trifluoroacetic acid, to obtain and assign a reference 2D 1H–15N HSQC spectrum for the H/D exchange analysis. Here, we report the near-complete assignments of backbone and ...
Partial, edited 2D IPAP (HNCO)-(J-CA)NH spectra (Fig. 2A) acquired with an aligned sample show a good separation of the two lines of the Cα–Hα doublet. Clearly, the crowded nature of the 1H–15N HSQC spectrum for this α-helical protein meant that without the extended 15N ...
HSQC (heteronuclear single quantum coherence) 13C–1H correlation spectra of whole biomass in perdeuterated pyridinium chloride–DMSO system: An effective ... "HSQC (heteronuclear single quantum coherence) 13C-1H correlation spectra of whole biomass in perdeuterated pyridinium chloride-DMSO system: An...
These molecules were thoroughly characterized by IR, 1H, 13C, 15N, 2D-NMR (COSY, NOESY, 1H-13C, 1H-15N HSQC and HMBC), and X-ray diffraction. NMR studies revealed the existence of conformational/configurational isomers around the amide and imine functionalities. The major species in DMSO ...