38 When you go on your job interview(面试),be sure to dress well, be on time, do your research and NEVER ask any of the following questions : 1." How quickly could I be promoted(晋升)" An employer first of all wants an employee to be happy with the position they have. Ambition(...
Cells were seeded at a density of 30,000 per well and grown to confluency overnight on glass coverslips in 24-well tissue culture dishes (Corning B.V., The Netherlands). Labelled exosomes were then added at a dose of 2.0× 1010 particles per well and incubated for 1, 4, 12 and 24 h...
cascades and the AKT1-GSK3 signaling pathway is an appropriate objective for future translation researches. In line with the observed behavioral amelioration inAkt1+/−female mice treated with lithium, the injection of lithium increased pGsk3α/β (Ser21/Ser9) expression in WT controls as well ...
A total of 100 μl of cell suspension was added to each well of the Matrigel- precoated 96-well plate, and the tubes that formed were imaged and counted under a microscope. For aortic ring assays, the thoracic aortas of 8-week- old C57BL/6 mice were processed according to a previ- ...
The next day, the samples were detached from the culture dish and transferred to a 12-well plate with differentiation medium. Medium was changed every day thereafter. Extending Angle and Straightness of Neurite-like Processes At the end of the culture period of 7 days, the samples were washed...
46 (smoke) does harm to other’s health as well as to that of the smokers. Besides, about 10% of air pollution is caused by other reasons. We should take some 47 (measure) to fight against pollution. New fuel can be used to take __ 48 _ place of gas. We can plant more trees...
The cells growing in culture vessels were detached and seeded on 96-well plates at a density of 6 × 104 cells/well with 200 μL RPMI 1640 containing 10% FBS, 1% penicillin–streptomycin, with/without the XF (0.5 or 1 μg/mL) in a 96-well plate and were incubated at 37 °C. 24...
Average length and density of neurites were evaluated based on microscopic images 24 h after seeding (day 0) and then on days 2, 3, 5, 7, 14, and 21 of incubation with 100 ng/mL rat NGF. In these assays, cells were seeded with reduced density (1000 cells/well). Cell differentiation...
仪器外观 HydroBooster SensorCartridge CartridgeLid 耗材 自动药物注射端口启用实时功能分析 CellMitoStressTestAssayKits 实验前一天 水化探针 1.在UtilityPlate每孔中加入1ml校准液 (SeahorseXF校准液用于溶解和校准XF小柱，并包含FluxPak)2.将HydroBooster放在UtilityPlate上，SensorCartridge放在最上面 3.放入无CO2的培养...
well as the soybean yield was carried out through a geostatistical mesh. During the soybean growth, we carried out four applications of insecticides for pest control. In the end of the cycle, the soybean grain yield was determined in an area of 3.24 m2 located around the sampling point. ...