Primers of cDNA PCR amplification 表选项 下载CSV 1.2.2 细胞培养与表达质粒的转染 HEK293细胞培养液为DMEM完全培养基,其中含有10%胎牛血清和1% Penicillin-Streptomycin(PS)溶液,培养箱环境为37 ℃和5% CO2。细胞在转染前12 h接种到细胞培养板中,24孔细胞培养板以12万个/孔细胞量接种,6孔细胞培养板以...
All media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin antibiotic solution. Cells were maintained in 5% CO2 in a humidified atmosphere at 37 °C. Plasmids were transiently transfected into mammalian cells using TurboFect (Thermo Scientific, Waltham, MA, USA)...
HaCaT human keratinocytes were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 1% penicillin/streptomycin (Gibco) and 10% fetal bovine serum (FBS, Gibco). Cells were incubated at 37 °C and 5% CO2. PC device The new type of pla...
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All mediums are supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin/streptomycin (P/S; Thermo Fisher Scientific). All cells were cultured in a 37 °C humidified incubator containing 5% CO2. RNA extraction and quantification RNA purification was performed ...
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PCa cell lines, including PC3, DU145, and 22RV1, were cultivated in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Yesean, Shanghai, China). Human normal prostate epithelial cells (RWPE-1) were cultured in a Defined ...
BI 03-031-1B Penicillin-Streptomycin Solution青链霉素混合液(双抗) 价格电议 起批量≥1 瓶 最小起订1瓶 供货总量100瓶 发货地址广东省广州市 建议售价￥电议 更新日期2025年01月17日 产品规格100 毫升 即时洽谈立即询价查看联系方式 收藏产品发送留言 ...
Raw264.7 cells and BHK21 cells were maintained in Dulbecco's Modified Eagle's medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS; Gibco) and penicillin-streptomycin. RAW-Blue cells (InvivoGen) were maintained in DMEM supplemented with 10% FBS and 100 μg/ml Normocin. ...
Strains of streptococci and enterococci showing moderate resistance (MIC 6–500 mg/L) exhibit synergy with penicillin, but strains showing high levels of resistance (MIC >500 mg/L) have ribosomes that are resistant to streptomycin and simultaneous treatment with penicillin is without effect. ...