Teddington, UK). All cells were routinely cultured in RPMI 1640 (Life Technologies 11965), or Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FBS recommended by suppliers. The cell lines were authenticated by short-tandem-repeat (STR) analysis and matched to the German Collec...
(Gibco) containing 0.5% glucose, 2% FCS, 1 mM Na-Pyruvate, 0.01 M HEPES pH 7.35, 1% Penicillin-Streptomycin, 1% B27 supplement and 1% Glutamax, tissues were triturated using a 1 ml pipette until fully dissociated. The cell suspension was diluted further with complete BME media and...
克霉唑 23593-75-1 PENICILLINSTREPTOMYCIN,100X青霉素, 链霉素溶液,100X组织培养级透明液体FROZENsigma GLYCINE甘氨酸蛋白组学级白色结晶粉末RTsigma TRIS-GLYCINEBUFFER,10XSOLUTIONTris-甘氨酸缓冲液蛋白组学级透明无色液体COLDsigma TWEEN®80吐温80蛋白组学级金黄色-黄色粘稠液体RTsigma 公司是一家专注于生命科学和...
In brief, femurs and tibiae were isolated from mice, ensuring that joints were kept intact, and placed in cold DMEM (Cat: 11,995, Gibco) containing 1% penicillin/streptomycin (P/S). The muscle was removed using a sterile scalpel and scissors, the ends of the bones were cut in a ...
Tissues were cultured in E7 medium: DMEM supplemented with 20% FBS (Gibco, Catalog No. 16000), 1% nonessential amino acid, 2 mM glutamine, 1% penicillin–streptomycin, 100 μM mercaptoethanol. Outgrowth of fibroblasts was observed between 5 and 10 days. 2.2. Lentivirus transduction To generate...
The TPC1 and K1 cells were cultured with RPMI1640 medium (RPMI1640; Gibco, USA) supplemented with 1% penicillin - streptomycin (penicillin: 100 U/mL and streptomycin 100 μg/mL; Enpromise, Hangzhou, China) and 10 % fetal bovine serum (Gibco, USA) in a humidified atmosphere at 37 °C ...
cell line AGS was cultured in F12K medium, while the rest of the cells were cultured in RPMI 1640 medium. Both media were supplemented with 10% foetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Gibco, USA). Cells were incubated at 37 °C in a humidified atmosphere of 5% ...
英文名称:Penicillin-Streptomycin Solution,10000 units per ml Penicillin G Sodium Salt,10mg per ml Streptomycin Sulfate 产地:以色列 品牌:BI 包装规格:100ml 货号:03-031-1B 产品描述: 青霉素-链霉素是一种氨基糖苷类-β内酰胺类抗生素联合溶液。它对革兰氏阳性和革兰氏阴性细菌具有广谱杀菌活性。青霉素G的作用...
Penicillin-Streptomycin, liquid (青链霉素双抗) 10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline 100 mL 15140-122 15140122 MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140...
(Hyclone). PANC-1, MIA-PaCa-2, AsPC-1, PANC-28, BxPC3 were grown in DMEM (Hyclone). All media were supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin. All cells were maintained at 37 °C in a humidified atmosphere with 5% CO2except SW1990 which ...