HEK293细胞培养液为DMEM完全培养基,其中含有10%胎牛血清和1% Penicillin-Streptomycin(PS)溶液,培养箱环境为37 ℃和5% CO2。细胞在转染前12 h接种到细胞培养板中,24孔细胞培养板以12万个/孔细胞量接种,6孔细胞培养板以50万个/孔细胞量接种;转染操作严格按照Mega Tran1.0说明书进行,对HEK293细胞转染效率约为95%...
Penicillin-streptomycin solution was purchased from Biological Industries (Beit HaEmek, Israel). Culture dishes were maintained in a humidified atmosphere under standard conditions of 5% CO2 at 37°C. For hypoxic conditions, cells were incubated in hypoxia chamber with 1% O2 concentrations. STR DNA ...
1640 medium, 10% (v/v) human AB serum and 1% (v/v) penicillin–streptomycin for 45 min. Overlapping peptide pools were plated at 4 µg ml−1, 50 µl per well; dimethylsulfoxide (Sigma) was used as the negative control at the equivalent concentration to the peptides. A...
培养基:10% FBS、DMEM/F12、1% Penicillin-Streptomycin 换液频率:每2-3天换液一次。 组织来源:人脐带(江苑生物人脐带间质干细胞取自健康足月分娩孕妇的脐带,拥有强大的自我更新能力并具有多向分化潜能。) 生长特性:贴壁生长 细胞形态:成纤维细胞样
100×青霉素-链霉素-庆大霉素混合液 价格: ￥168/块 产品详情:100×青霉素-链霉素-庆大霉素混合液 100 mL SL6042-100mL 酷莱博 168 N/A 128 Penicillin-Streptomycin-Gentamycin Solution 包装规格:100 mL 货号:SL6042-100mL 品牌:酷莱博 产地:国产 是否进口:否 青霉素-链霉素-庆大霉素混合溶液(100×三抗)...
The TPC1 and K1 cells were cultured with RPMI1640 medium (RPMI1640; Gibco, USA) supplemented with 1% penicillin - streptomycin (penicillin: 100 U/mL and streptomycin 100 μg/mL; Enpromise, Hangzhou, China) and 10 % fetal bovine serum (Gibco, USA) in a humidified atmosphere at 37 °C ...
PBMC were isolated from non-cancer controls by density gradient centrifugation and seeded at a density of 1 × 106 cells/mL RPMI supplemented with 10% foetal bovine serum (FBS) and 1% penicillin-streptomycin (cRPMI). To determine whether CX3CR1 was internalised via endocytosis following treatme...
(American Type Culture Collection, Rockville, MD, USA) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) in a humidified incubator with 5% CO2 at 37 °C...
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