Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uLwere then transferred to LRP5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. Aft...
R22010-100 常规溶液 5%BSA封闭液 100ml R22010-500 常规溶液 5%BSA封闭液 500ml R22088-500 常规溶液 1×PBST缓冲液(0.01M,pH=7.2-7.4) 500ml P6121-500 常规溶液 10×PBS缓冲液(pH=7.2-7.4) 500ml P6122-500 常规溶液 10×PBST 500ml T16641-500 常规溶液 10×Tris-Tricine-SDS缓冲液 500ml T16440...
英文名称:1 x Western Block Buffer in PBST with BSA 货号:XF-M1368 规格:400ml 储存条件:2-8℃ 有效期:12个月产品描述:WB 封闭液(BSA,1 x in PBST)可以用于Western时转移了蛋白样品的PVDF膜或NC膜的封闭。作用原理:Western Blot牛血清白蛋白封闭液的作用原理在于占据非目标结合位点,从而降低背景信号,提高...
储存液0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% 产品应用WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 抗体制备过程: 1.材料与试剂 a.提取的动物Ig b.弗氏完全佐剂和弗氏不完全佐剂 ...
方法1:我们的抗体(冻干粉)已经包含了0.01M PBS、1% BSA和0.1% 防腐剂(叠氮钠或庆大霉素),您只需要加入灭菌的双蒸水就行了。抗体溶解后,置于-20℃保存,*分装后使用,避免反复冻融,可保证至少1年有效; 方法2:也可以只加入一半体积的双蒸水,再用一半体积的甘油补足,置于-20℃保存,这样抗体不会冻,有利于抗体...
R22010-100 常规溶液 5%BSA封闭液 100ml R22010-500 常规溶液 5%BSA封闭液 500ml R22088-500 常规溶液 1×PBST缓冲液(0.01M,pH=7.2-7.4) 500ml P6121-500 常规溶液 10×PBS缓冲液(pH=7.2-7.4) 500ml P6122-500 常规溶液 10×PBST 500ml T16641-500 常规溶液 10×Tris-Tricine-SDS缓冲液 500ml T16440...
R20405-500 通用Buffer Tris-BSA溶液(0.02M,pH=7.0) 500ml R20406-500 通用Buffer Tris-BSA溶液(0.1M,pH=7.0) 500ml C0403-100 通用Buffer Triton Lysis Buffer 100ml C0403-500 通用Buffer Triton Lysis Buffer 500ml R21908-15 通用Buffer Washing/Dilution Buffer 15ml R20613-100 通用Buffer XB盐溶液(20...
Incubated cells with 1% BSA, 22.52 mg/mL glycine in PBST (PBS + 0.1% Tween 20) for 30 min to block unspecific binding of the antibodies. Incubated cells in the diluted FUS antibody in 1% BSA in PBST in a humidified chamber for 1 h at room temperature. Decanted the ...
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
储存液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 产品应用WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. ...