Make 1% low melting temperature agarose in isotonic buffer, heat to melt the agarose then cool to 37°C. Warm the cell suspension to 37°C, mix 1:1 with the melted agarose and dispense into appropriate molds. It is best to keep the molds on ice so that the agarose will gel rapidly...
To make a precise scientific or clinical statement based on your Immunofluorescence staining you must ensure the specificity of your primary antibody to its target antigen. In doing so you should not exclusively rely on the statements of your commercial providers. Choose your antibody according to al...
In regards to western blotting, a common question we always get is, what should I use, milk or BSA to block? So the purpose of the blocking step is to reduce the amount of background due to non-specific bonding. Now, BSA is only made up of one protein, BSA at 60 kDa, whereas mi...
And learning how to make banners together with the BannerBoo is incredibly simple. Check it out: Step 1. Log in to the site; Step 2. Choose the required banner size (choose from pre-made sizes or create an individual size for your website or advertising platform); Step 3. Start...
How to Make Contract More Binding
Make arrangements to have the money transferred from your bank account to the seller. How to handle questions from your bank If you specifically need cash, and you have no other option but to withdraw $10,000 (or more) of cash in one day, be prepared to explain to y...
Since we make use of spPCR, a perfect unidirectional orientation of the DNA molecules with a 5′ (surface) to 3′ direction can be ensured. Other photolithographic methods are also available with which such an orientation can be achieved, although a 5′-3′ orientation is not standard19,20...
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The molecular weight of BSA is 35,000 g/mol. You have a 2% solution of BSA. You want to make 0.25 mg/ml of BSA, and you need 1 mL of this diluted solution. How much BSA and how much water will you use? An overn...
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