While preservation and subsequent visualization of enzyme activity in soft tissues can be complicated, it is particularly difficult in bone specimens, especially those that have been decalcified. For these studies, we examined the bones of parathyroid hormone-related peptide (PTHrP) knock-in mice in...
英文: Rapid Detection of Beta-galactosidase Activity by Test Paper中文: 用试纸法快速检测β-半乳糖苷酶活性的研究 英文: Cloning and expression of the beta-galactosidase gene of Paenibacillus sp.K1 in E.coli中文: Paenibacillus sp.K1 β-半乳糖苷酶基因的克隆及在大肠杆菌中的表达 ...
While preservation and subsequent visualization of enzyme activity in soft tissues can be complicated, it is particularly difficult in bone specimens, especially those that have been decalcified. For these studies, we examined the bones of parathyroid hormone-related peptide (PTHrP) knock-in mice in...
If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity. Formulation The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5). Specific Activity One unit of ...
One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact gram-negative bacteria, yeast, and mammalian cells. Bertoni,G.One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact gram-negative bacteria, yeast, and mammalian...
Choose stand-alone assay reagent or complete kit for cell lysis and detection Colorimetric assay easily measured in a plate reader or spectrophotometer (405 nm) Adaptable to 96-well microplates or tube assay formats Perform homogeneous screening assays or measure activity secondarily after cell lysis ...
and having activity to transfer the resultant D-galactose group; optimum pH: 7.0-7.5; stable at pH6.5-7.5 (after treatment at 40°C for 60min); optimum temperature for activity: 30-40°C; and molecular weight (determined by gel filtration method): 320000.;COPYRIGHT: (C)1996,JPO...
beta ‑galactosidase activity. GM1 gangliosidosis demonstrates varying degrees of clinical severity but is invariably fatal, and children with the most common and severe form of GM1 gangliosidosis usually die within 3 years of birth. Morquio B syndrome patients are neurologically normal, but display ...
beta-Galactosidase activity of the jejunum and ileum of newborn, suckling and weaned rats; comparison of activities of beta-galactosidase towards different... activity of the jejunum and ileum of newborn, suckling and weaned rats; comparison of activities of beta-galactosidase towards different substr...
Any skilled person will recognize that an enzymatic activity assay, immunoassay, western blotting and other detection assays can be used to detect at the protein level, the presence or absence of the proteins which the isolated sequences encode for. At DNA level, Southern blotting, northern blotti...