Define Alpha-Amylase. Alpha-Amylase synonyms, Alpha-Amylase pronunciation, Alpha-Amylase translation, English dictionary definition of Alpha-Amylase. n. A form of amylase in the saliva of humans and certain other animals that catalyzes the hydrolysis of
4. The intracellular localization of beta-galactosidase, alpha-mannosidase, beta-N-acetylglucosaminidase and alpha-L-fucosidase in mammalian tissues. Biochem J 87: 354–361. Deugnier Y, David V, Brissot P, Mabo P, Delamaire D, Messner M, Bourel M, Legall JY (1984) Serum alpha-L-fuc...
The luciferase and beta-galactosidase were measured following the protocol provided by the kits. The bar represents standard deviation of three independent experiments Download: Download high-res image (36KB) Download: Download full-size image Fig. 4. UAP56 interacts with and promotes cytosolic mRNA...
The membranes were 136–452 (ILK-C)18 or b-galactosidase (bGAL) were then briefly washed and incubated in primary grown in 293 cells and purified by CsCl2 ultra- antibody diluted in PBS-T with 2% milk for 1 h. centrifugation or used as cell lysate supernatants. Primary antibodies ...
Luciferase assays were performed using Luciferase Assay reagent (Promega, USA) and β-glycosidase assays were performed according to the β-galactosidase Enzyme and Assay System with Reporter Lysis Buffer Kit (Promega, USA). Luciferase expression values were normalized by comparison with β-glycosidase ...
After washing non-specific particles enzymes labeled anti-IgE (mouse monoclonal β-galactosidase-anti-IgE) are added to form the complex. Following incubation, unbound conjugate (enzyme-anti-IgE) is washed away, and the bound complex is then incubated with a developing agent (4-methylumbelliferyl-...
Cardiac myocytes were transfected with a mouse PGC-1α luciferase reporter construct PGC1αluc, as previously reported [20,21,22]. To normalize differences in transfection efficiency, co-transfection with β-galactosidase or Renilla expression vector (pRL, 5 ng; Promega, Madison, WI, USA) was ...
Transfected cells were exposed to normoxia (N, open bars) or hypoxia (H, shaded bars) for 16 h and then assayed for luciferase and β-galactosidase activities. The constituent plasmid(s) in each transfection experiment is/are indicated by a + sign underneath the respective bar charts. ...
Chen HT, Lin MS, Hou SY: Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production. Kor J Chem Eng. 2008, 25: 1082-1087. 10.1007/s11814-008-0177-0. Article CAS Google Scholar Sambrook J, Russell DW: Molecular cloning: a laboratory manual. 2001, ...
A major reason for the failure of the immune system to detect tumor antigens (TAs) is the insufficient uptake, processing, and presentation of TAs by antigen-presenting cells (APCs). The immunogenicity of TAs in the individual patient can be markedly inc