The capability to manipulate and alter the various organisms has been one of the supreme achievements of modern molecular biologists. Custom designed zinc finger proteins (ZFPs) provide a powerful platform technology since other functional domains like non-specificFokI cleavage domain (N), transcription...
B Full length Rag1 protein consists the functional important zinc-binding dimerization domain (ZDD) containing the RING finger (RING) and the zinc finger A (ZFA) as well as the c-terminal core domain with the nonamer-binding region (NBR) and the zinc finger B (ZFB). Mutated Rag1 lost...
To determine the subcellular localization of the two ZFPs, VdZFP1 and VdZFP2 were fused with the green fluorescent protein (GFP) and transferred into the highly-virulent strain AT13 (wild-type (WT) strain). Surprisingly, both were present in the cytoplasm of swollen hyphae and germinating co...
This class of peptides is involved in several cellular processes, and they can be found in proteins as a single functional domain, coupled with other zinc fingers or associated with other independent domains [45,46,47]. The consensus sequence (Y/F)-X-C-X2,4-C-X3-X-L-X2-H-X3,4-H...
GATAD1 (GATA ZNF domain-containing protein 1, also known as ODAG, ocular development-associated gene) contains a ZNF domain at the N terminus12, and is located on chromosomal region 7q21–q22 (ref. 13). GWAS have implicated GATAD1 as a gene associated with adult height14,15. GATAD1 ...
As used herein, a “fusion protein” refers to a polypeptide with covalently linked domains as well as a complex of polypeptides associated with each other through non-covalent bonds. The transcription repressor domain can be associated with the ZFP domain at any suitable position, including the ...
www.nature.com/scientificreports OPEN received: 28 January 2016 accepted: 13 May 2016 Published: 02 June 2016 The N-terminal zinc finger domain of Tgf2 transposase contributes to DNA binding and to transposition activity Xia-Yun Jiang1,2,*, Fei Hou1,*, Xiao-Dan Shen2, Xue-Di ...
Proteasome-associated control elements (PACE) are found upstream of the corresponding genes. B, Cuz1 is evolutionarily conserved, containing an AN1-type zinc finger (Zf_AN1) domain in its N-terminal region. Aligned proteins were chosen based on the phylogenetic tree of Cuz1 orthologs from the...
Aims Kr眉ppel-like factor 11 ( KLF11 ) is a transcriptional factor of the zinc finger domain family that regulates the expression of insulin. In North Eur... T Tanahashi,K Shinohara,P Keshavarz,... - Wiley-Blackwell 被引量: 11发表: 2008年 Expectations from the cessation of vaccination...
Each ZF domain primarily contacts three base pairs (bps) within the major groove of DNA. However, ZFs can be modified to recognize a broad range of DNA sequences4,6. Ideally, a pair of ZFNs is designed for a particular target, whereby dimeri- zation of the associated cleavage domains is...