Zinc-finger proteins (ZNFs) are one of the most abundant groups of proteins and have a wide range of molecular functions. Given the wide variety of zinc-finger domains, ZNFs are able to interact with DNA, RNA, PAR (poly-ADP-ribose) and other proteins. Th
[VIR])8,11, and HAKAI (also known as Casitas B-lineage lymphoma-transforming sequence-like protein 1 (CBLL1), plant homolog HAKAI)8,11, and in animals it also contains and requires RNA binding motif protein 15 (RBM15) and the zinc finger CCCH domain-containing protein 13 (ZC3H13)12,...
Zinc Finger Nucleases (ZFNs) are a programmable DNA binding protein system composed of zinc finger proteins fused to a bacterial nuclease. They enable site-specific double stranded DNA breaks for gene editing by recognizing specific DNA sequences within the genome. ...
An abstract is unavailable.doi:10.1097/01.NT.0000460878.58028.82HurleyDanNeurology Today
antisense TE sequences that hybridize with the TE RNA in the germ line and the soma resulting in the degradation of the TE RNA.17–19 Both of these mechanisms, the purging of deleterious insertions of TEs by means of natural selection and the production of repressors using antisense sequence...
Conceptually, the accumulation of zinc-finger (ZF) motifs for high-affinity binding is similar to that of transcription factors (TFs) that are composed of ZF arrays. For example, the essential insulator protein CTCF has eleven ZFs29, and KRAB-ZF proteins feature twelve ZFs on average30. The ...
ZNF268b2 consists of only the zinc finger domain and has been reported to be an IκB kinase (IKK)-associated candidate of the NF-κB signaling pathway (4). The ZNF268 promoter is located in the first exon of the gene, and cAMP-response element-binding protein 2 (CREB2) binds the ...
The ZFP domains of the present disclosure may be fused to a transcription factor to repress the transcription of the mammalian PRNP gene into RNA. The fusion proteins are called zinc finger protein transcription factors (ZFP-TFs). These ZFP-TFs comprise a zinc finger protein (ZFP) domain that...
The mRNA is isolated, and cDNA is produced and used as a template to make antisense RNA (aRNA). The aRNA is amplified in the presence of fluorescent nucleotides, and labeled probes are incubated with the microarray so that the probe sequences hybridize to complementary oligonucleotides of the ...
Zinc finger domains are involved not only in DNA recognition, but also in RNA binding and in protein-protein binding. Current estimates are that this class of molecules will constitute about 2% of all human genes.Most zinc finger proteins have conserved cysteine and histidine residues that ...