OBJECTIVE :To investigate the interaction of long non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1) in secondary cerebral edema (CE) and neuron injuries after traumatic brain injury (TBI) in a mouse model. METHODS :TBI mouse models was established by free-fall strike. Adeno-associated ...
Given the wide variety of zinc-finger domains, ZNFs are able to interact with DNA, RNA, PAR (poly-ADP-ribose) and other proteins. Thus, ZNFs are involved in the regulation of several cellular processes. In fact, ZNFs are implicated in transcriptional regulation, ubiquitin- mediated protein ...
Zinc-finger proteins (ZNFs) are one of the most abundant groups of proteins and have a wide range of molecular functions. Given the wide variety of zinc-finger domains, ZNFs are able to interact with DNA, RNA, PAR (poly-ADP-ribose) and other proteins. Th
17.4.2Gene editing with programmable nucleases: Zinc finger nuclease The ZFN was the first programmable syntheticnucleaseof its kind. The protein consists of a DNA-binding domain on the N-terminus and a cleavage domain on the C-terminus. The DNA-binding domain contains three to sixzinc finger ...
The redox stability of Zn-binding protein domains makes them highly suited for nucleotide-binding and protein–protein interactions, with notable examples including the Zn-finger domain proteins, which is the largest class of Zn-binding proteins (Laity et al., 2001). However, the redox stability ...
Two of the clones contained partial cDNAs for a novel zinc-finger protein which we call EZF. A λZAP library was then constructed from poly(A)+ RNA of E15.5-day whole mouse embryos and screened with the insert from one of the EZF clones isolated above. Three positive clones of 2.5 to...
Recently, CtBP-interacting BTB Zinc Finger Protein (CIBZ) was identified as a key transcriptional regulator of ESCs and was shown to play a role in G1/S transition partly depending on Nanog expression (Nishii et al., 2012). Moreover, ZFP57 was shown to maintain the genomic imprints in ...
Here we show that viral integrations at Evi3up-regulate the expression of a novel Krüppel-like zinc finger protein via 3′ LTR-directed promoter insertion. EVI3 has high homology with EBFAZ, a multifunctional protein that participates in many signaling pathways via its multiple zinc fingers.8 ...
In C2H2-type zinc finger proteins, since the determination of the first structure reported for a three-finger protein in complex with DNA more than 25 years ago (Pavletich and Pabo, 1991), the DNA recognition process is sufficiently understood to define a DNA recognition code (Choo and Klug,...
Crozatier et al., “Single Amino Acid Exchanges in Separate Domains of the Drosophila serendipity δ Zinc Finger Protein Cause Embryonic and Sex Biased Lethality,” Genetics, 131:905-916 (1992). Crystal R.G., “Transfer of Genes to Humans: Early Lessons and Obstacles to Success,” Science...