Antigen retrieval was performed by microwaving in citrate solution for 10 min; then, the slides were blocked with 10% goat serum and incubated with anti-YTHDF2 antibody at 4°C overnight. The next day, the slides were washed in PBST (0.1% Tween-20 in PBS) three times to remove ...
After each antibody incubation, the membranes were thoroughly washed five times with PBST (PBS + 0.1% Tween-20) to remove unbound antibodies. Protein detection was carried out using an enhanced chemiluminescence (ECL) system (Thermo Fisher; #A38554), and the signals were captured with the ...
antibody抗体(SA00001‑1,Proteintech),抗rabbit IgG(H+L)‑HRP antibody抗体 (SA00001‑2,Proteintech);抗EIF3F抗体(G0815,Santa Cruz Biotechnology);FLAG (M185‑3,MBL);抗purinomycin抗体(MABE343,EMD Millipore). 8 8 CN117535410A说明书7/14页 ...
IP was performed with the addition of an ATM antibody (sc-377,293, Santa Cruz) or with control IgG and protein A-sepharose beads; the mixes were incubated overnight at 4 °C with gentle rotation. Beads were then washed with lysis buffer three times, and the supernatants were harvested ...