Macrophages were detected by immunostaining using rat anti-mouse F4/80 antibody (1:150, Serotec) as described above. Similarly, a segment of L5 spinal nerve containing the dorsal root ganglia, as well as, the dorsal and ventral roots was removed from female untreated SOD1G93A mice (n =...
Antibody/protein complexes were captured using protein A Sepharose, eluted in 2 × SDS sample buffer, run through Bis/Tris gels, transferred onto PVDF membranes, and probed with a polyclonal antibody raised against SOCS3. Membranes were reprobed with an antibody to HSP-70 (Santa Cruz ...
AntibodySourceCatalog #Dilution used Goat anti-Rabbit IgG – IRDye 800CW Li-Cor Biosciences 926–32211 1:10,000 Goat anti-Mouse IgG – IRDye 680LT Li-Cor Biosciences 926–68020 1:10,000 (W, Western blot; F, flow cytometry; CST, Cell Signaling Technologies; TFS, Thermo Fisher Scientific...
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