A simple way to express a protein with a leader sequence is to have the leader sequence encoded in the destination vector. The other option is to have the leader sequence subcloned into the entry vector using restriction enzymes, or incorporate the leader sequence into the forward PCR primer ...
Pack size: 100μg 1μg Price: 888.00RMB •Purpose Express target protein with C-terminal YFP tag in mammalian cell •Depositor Beyotime •Publication Lin et al. RNA Biol. 2019 May;16(5):626-638. •Sequence Information D2630 pCMV-C-YFP-seq.txt For Details Add to cart Brief ...
PRODUCT CAT.# DESCRIPTION SIZE PRICE *50% discount on the second and subsequent vectors encoding same fluorescent protein or sensor ordered by the same customer.The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and del...
The Thy1-Yellow Fluorescent Protein-H (Thy1-YFP-H) transgenic mouse (Jackson Laboratories, ME) expresses YFP in only 0.2% of RGCs. This reduced expression enables single RGC imaging with visualisation of the entire dendritic arbour and provides valuable clues on the impact of experimental ...
Taking advantage of the phenomenon of bioluminescence resonance energy transfer (BRET), we developed a bioluminescent probe composed of EYFP and Renilla reniformis luciferase (RLuc)€"BRET-based autoilluminated fluorescent protein on EYFP (BAF-Y)€"for near-real-time single-cell imaging. We show tha...
YFP-Parkin catalog : 23955 more info or order: Addgene product webpage citations: 33 Reference Kocaturk N, Peker N, Eberhart K, Akkoç Y, Deveci G, Dengjel J,et al. Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto ...
Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the ...
backbone_size promoter sequencing_primer_3 sequencing_primer_5 vector_types Other Gateway growth strain : Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system ...
Intense excitation of the protein in vitro for extended periods can generate free radicals that are quite toxic to cells [15]. The inability to obtain constitutively expressing GFP cell strains may also be related to DNA methylation effects. In the presence of an irreversible inhibitor of methyl ...
Here we took advantage of the mouse strain B6.Cg-Tg(Thy1-YFP)16Jrs/J (Thy1 YFP-16), where yellow fluorescent protein (YFP) is under control of the promoter of Thy1 gene. This allows visualising whole neurons, i.e. their cell body, axons and dendrites. In this work fluorescent cells...