Sugimura, T., Okabe, K., Imamura, A.: Number of cytoplasmic factors in yeast cells. Nature (Lond.) 212 , 304 (1966)SUGIMURAT,., K. OKABEand A. IMAMUR19A6,6 Number of cytoplasmic factors in yeast cells. Nature 212: 304.Number of cytoplasmic factors in yeast cells - SUGIMURA, ...
Using single-molecule fluorescence in situ hybridization, we count the number of transcripts of a enzyme in single cells during adaptation. The variation of response times between cells is small, yet we find a high transient variability in the copy numbers. Surprisingly, single cells display ...
The average value or the number distribution of any intracellular property captured by the individual cell model can be calculated by simulation of a sufficient number of individual cells. The proposed method is applied to a simple model of yeast glycolytic oscillations where synchronization of the ...
The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The tradition
Monolayers of HeLa cells were incubated with Candida suspensions and the number of adherent yeasts per unit area of epithelial cells counted microscopically. Adhesion was proportional to the incubation time and the yeast concentration in the suspension. Preincubation of Candida with sucrose facilitated the...
Cells from each of the 21 mutant libraries were spread on YPD and YPG plates, followed by colony counting after growth. Respiration is needed for cell growth on YPG but not on YPD. b, Mean ratio of YPD colony number to YPG colony number for each mutant library, based on three replicates...
This differential sensitivity of vegetative cells can be exploited to advantage in a number of genetic manipulations. These include: random spore analysis; selecting mutants affected in the developmental processes of sporulation and spore germination; detection of sporulating revertants or recombinants from...
The number of cells was directly counted using a hematocytometer. Total RNA was extracted using a hot-phenol method [12] with some modifications. To the 400-μl cell suspension described above, 100 μl of 5× lysis buffer (50 mM Tris-HCl, pH 7.5/50 mM EDTA/2.5%(w/v) SDS) and ...
Quantification of number of cells with nuclear GFP was carried out using FIJI. Full size image Next, we examined the role of Snx4/Atg24, a sorting nexin that plays a role in proteasome autophagy upon nitrogen starvation24. Interestingly, α4-GFP atg8Δ cells used by Nemec et al. formed ...
Node size indicates the number of binding events for that TF. Edge width represents the number of cooperative or antagonistic events involving a specific TF-pair. g Overlap of NF-κB and AP-1 pY1H interactions with the literature. Numbers above each bar reflect the number of binding events ...