The other one was Y2= 0.147X2+0.137 (R2=0.984) , where X2 was the OD600 value, and Y2 was the number of viable cells(x 106 / ml). The bacterial counts of Yeast and Zymomonas mobilis can be got quickly and accurately by measuring the OD values, calculating from the two equations ...
Here, we present a regulatory protein of the ERMES complex, i.e., Emr1 (ERMES regulator 1), in the fission yeastSchizosaccharomyces pombe. The absence of Emr1 significantly decreases the number of ERMES foci and Emr1 interacts and colocalizes with the ERMES core components Mdm12. Hence, Em...
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activi
The first actin binding protein identified in yeast, Abp1 functions in actin-based endocytosis in yeast and in receptor-mediated endocytosis in mammalian cells. To explore the functions for Abp1 in Dictyostelium, we examined the phenotypes of cells that overexpressed the Dabp1 protein and cells ...
a higher OD than the culture with the analogous high-copy plasmids. This higher OD increased the overall yield of the desired products in the low-copy plasmid system. Thus, more cells producing a smaller amount of compound can be more productive than fewer cells producing a lot of the ...
Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and rapid quantificati...
Experiments are needed to evaluate the functional role of additional ATG genes in flower senescence. Autophagy was first discovered in yeast as an adaptive mechanism of cells under nutrient starvation [20]. It is now known in many plant species that autophagy not only contributes to nutrient ...
from this screen, we were able to notably improve stable transformation in bothA. thalianaand the oleaginous yeastRhodosporidium toruloides. Thus, our work demonstrates the impact of binary vector backbone engineering on AMT across kingdoms, creating an easily deployable strategy to improve ...
thus resulting in mutant variants equipped with different fixed PCNs24,32,33. For instance, in NEBStableE. colicells the fPCN collection offers an approximately 20-fold PCN range with proportional gene expression levels of the reporter protein sfGFP (Fig.1b) without noticeable perturbation to gro...
versatile IRES used to initiate translation in both, mammalian and yeast cells50,51. All these regulatory elements were assembled using gene synthesis (GenScript) and 40 nucleotides of sequence homology to the insertion sites in the vector backbone (pESC-LEU, Agilent, cat #217452; pESC-TRP,...