Strains, plasmids, synthetic gene fragments and oligonucleotides The yeast GFP collection34 and the S. cerevisiae strain BY4742 were used for the creation of the essential GFP-ERdd library (Supplementary Data S1) and generation of direct ERdd fusion strains (Table S1), respectively. DNA constructs...
prepared fluorescently-labeled theonellamide A, and wrote the manuscript; P.N. carried out experiments and data analysis of the liposome leakage experiments, and wrote the manuscript; J.L.Y.K. provided computational support for MoBY-ORF validation, database construction and sequencing analysis; J....
(1997). BTN1, a yeast gene corresponding to the human gene responsible for Batten's disease, is not essential for viability, mitochondrial function, or degradation of mitochondrial ATP synthase. Yeast 13, 691- 697.Pearce DA, Sherman F (1997) BTN1, a yeast gene corresponding to the ...
YPL.db2: the yeast protein localization database, version 2.0 Yeast. 2005 Feb;22(3):213-8. Research Support, Non-U.S. Gov't M Kals,K Natter,GG Thallinger,... - 《Yeast》 被引量: 119发表: 2005年 加载更多 来源期刊 Yeast 研究点推荐 yeast protein localization database 引用走势 2010...
Master gene list is a database containing thorough information about those genes that are being traced by the plate analysis research. According to the gene list, the bottom-right colony in each plate denotes colony 1, and the top-left colony in that plate represents colony 384 (for a 16 ...
They contained an average of 30,000 single nucleotide polymorphisms (SNPs) and 63 deletion events, with >200 base pairs (bp) per strain [2]. The nonessential gene deletion collection contained a single deletion in every gene, which highlighted the fact that approximately 50% of the deletion ...
Phenotypes are variable within species, with high phenotypic variation in the fitness and cell morphology of natural yeast strains due to genetic variation. A gene deletion collection of yeast laboratory strains also contains phenotypic variations, demon
We describe the cloning and analysis of TAF25, a previously uncharacterized yeast gene that encodes a yeast TATA-binding protein-associated factor or yTAF of Mr = 25,000. The gene encoding yTAF25 is a single copy essential gene, and the protein sequence deduced from TAF25 exhibits sequence ...
The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability. By screening a bank of temperature-sensitive mutants by anti-tubulin immunofluorescence microscopy, we previously identified the sad1.1 mutation (Hagan, I., ... Hagan...
Mutant expression profiles have been published for nearly all the nonessential regulators in yeast, yet there is a need for improved analysis and visualization tools to analyze these data and integrate it with complementary protein-DNA binding data. The RegulatorDB database contains mutant expression ...