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excised kidney samples and testicular tissue, which were fragmented and treated with a hypotonic solution (0.075 M KCl) for 20 min at 37 °C. Both mitotic cell suspensions and fragmented testes were preserved in Carnoy I fixative (3:1 methanol/glacial acetic acid). Chromosome slides were stai...
Struggling With Question Bank? Get Allen’s Free One Shot Videos Free ALLEN One Shots Text SolutionVerified by Experts The correct Answer is:CUpdated on:21/07/2023 | ShareSave Answer Step by step video, text & image solution for यदि y=(1+x)^(y)+sin^(-1)(sin^(2)x) ह...
( x3 − 3 x y ) dx + ( y − 3 x2 y ) dy = 0 Text Solution Verified by Experts The correct Answer is:C2(x2+y2)=y2−x2 | ShareSave Answer Step by step video, text & image solution for अवकल समीकरण (x^(3)-3xy^(2))dx+(y^(3)-3x^(...
Ym2 is highly related to Ym1 (GenBank M94584),18 the latter of which is also called eosinophil chemotactic factor (ECF-L, GenBank D87757),19 a chitinase-related protein. Ym1 and Ym2 share ∼95% identity but show tissue-specific expression; whereas Ym1 expression is high in lung and...
In short, protein samples were boiled for 10 min at 95 °C, electrophoresed in a 12% SDS polyacrylamide gel, transferred to PVDF transfer membranes (88520, Thermo Fisher Scientific) and blocked with fast blocking solution (PS108, Epizyme Biotech) at room temperature for 20 min. The ...
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1 mL of 40% Rochelle salt solution (potassium sodium tartrate) was added to the tubes. The tubes were then cooled down to ambient temperature, and the color intensity (dark red) was read at a wavelength of 510 nm using a spectrophotometer against distilled water (1 mL) as blank. The pro...
After drying at room temperature, 20 μL of DEPC water was added to obtain the RNA solution. The RNA concentration and purity were measured using a Nanodrop 2000 (Thermo Scientific, Shanghai, China), and the RNA solution was subjected to agarose gel electrophoresis to assess the RNA integrity...
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